Bifidobacterium longum increases serum vitamin D metabolite levels and modulates intestinal flora to alleviate osteoporosis in mice.

IF 3.7 2区 生物学 Q2 MICROBIOLOGY
mSphere Pub Date : 2025-03-25 Epub Date: 2025-02-21 DOI:10.1128/msphere.01039-24
Hongchao Wang, Gao Tian, Zhangming Pei, Xihua Yu, Yi Wang, Fuchun Xu, Jianxin Zhao, Shourong Lu, Wenwei Lu
{"title":"<i>Bifidobacterium longum</i> increases serum vitamin D metabolite levels and modulates intestinal flora to alleviate osteoporosis in mice.","authors":"Hongchao Wang, Gao Tian, Zhangming Pei, Xihua Yu, Yi Wang, Fuchun Xu, Jianxin Zhao, Shourong Lu, Wenwei Lu","doi":"10.1128/msphere.01039-24","DOIUrl":null,"url":null,"abstract":"<p><p>The elderly population is prone to osteoporosis, owing to the deterioration of the skin, liver, and kidney functions. Vitamin D (VD) supplementation has a limited effect, and VD deficiency is mostly treated with medication. Several studies have shown that the gut microbiota alters intestinal VD metabolism and that probiotic supplements can influence circulating VD levels. Therefore, in the present study, we screened a strain of <i>Bifidobacterium longum</i> FSHHK13M1 that can increase the level of VD metabolites in the fermented supernatant species <i>in vitro</i> by modeling fecal bacterial fermentation. The results showed that FSHHK13M1 intervention significantly increased the serum levels of 1,25-dihydroxy VD and osteocalcin. It activated the expression of the VDR, OPG, Wnt10b/β-catenin, and Runx2/Osterix pathways and inhibited the expression of RANKL/RANK pathway. Furthermore, there was an enhancement in the quantity of bone trabeculae and the proportion of bone volume. Concurrently, the gut microbiota in mice with osteoporosis exhibited signs of imbalance. FSHHK13M1 intervention increased the relative abundance of specific bacteria, such as <i>Faecalibaculum rodentium</i>, <i>Limosilactobacillus fermentum</i>, <i>Bifidobacterium pseudolongum</i>, and <i>Akkermansia muciniphila</i>. These results suggest that <i>B. longum</i> FSHHK13M1 alleviates retinoic acid-induced osteoporosis symptoms by modulating related genes, regulating the intestinal flora and increasing the level of active VD.IMPORTANCEOsteoporosis is a systemic metabolic disease in which the patient's bone mass decreases for a variety of reasons, and the microstructure of the bone tissue is altered, leading to an increase in bone brittleness and susceptibility to fracture. Osteoporosis is almost always present in the elderly population, and fractures from falls are an important predisposing factor for mortality risk in the elderly population. Supplementation is quite limited for them as they are not able to utilize vitamin D well due to declining liver, kidney, and skin functions. In the present study, a strain of <i>Bifidobacterium longum</i> probiotic was found to increase the levels of the active form of vitamin D and ameliorate osteoporosis. This may play an important role in preventing osteoporosis and reducing fracture risk in the elderly.</p>","PeriodicalId":19052,"journal":{"name":"mSphere","volume":" ","pages":"e0103924"},"PeriodicalIF":3.7000,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11934329/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"mSphere","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1128/msphere.01039-24","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/2/21 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

The elderly population is prone to osteoporosis, owing to the deterioration of the skin, liver, and kidney functions. Vitamin D (VD) supplementation has a limited effect, and VD deficiency is mostly treated with medication. Several studies have shown that the gut microbiota alters intestinal VD metabolism and that probiotic supplements can influence circulating VD levels. Therefore, in the present study, we screened a strain of Bifidobacterium longum FSHHK13M1 that can increase the level of VD metabolites in the fermented supernatant species in vitro by modeling fecal bacterial fermentation. The results showed that FSHHK13M1 intervention significantly increased the serum levels of 1,25-dihydroxy VD and osteocalcin. It activated the expression of the VDR, OPG, Wnt10b/β-catenin, and Runx2/Osterix pathways and inhibited the expression of RANKL/RANK pathway. Furthermore, there was an enhancement in the quantity of bone trabeculae and the proportion of bone volume. Concurrently, the gut microbiota in mice with osteoporosis exhibited signs of imbalance. FSHHK13M1 intervention increased the relative abundance of specific bacteria, such as Faecalibaculum rodentium, Limosilactobacillus fermentum, Bifidobacterium pseudolongum, and Akkermansia muciniphila. These results suggest that B. longum FSHHK13M1 alleviates retinoic acid-induced osteoporosis symptoms by modulating related genes, regulating the intestinal flora and increasing the level of active VD.IMPORTANCEOsteoporosis is a systemic metabolic disease in which the patient's bone mass decreases for a variety of reasons, and the microstructure of the bone tissue is altered, leading to an increase in bone brittleness and susceptibility to fracture. Osteoporosis is almost always present in the elderly population, and fractures from falls are an important predisposing factor for mortality risk in the elderly population. Supplementation is quite limited for them as they are not able to utilize vitamin D well due to declining liver, kidney, and skin functions. In the present study, a strain of Bifidobacterium longum probiotic was found to increase the levels of the active form of vitamin D and ameliorate osteoporosis. This may play an important role in preventing osteoporosis and reducing fracture risk in the elderly.

长双歧杆菌能提高小鼠血清维生素 D 代谢物水平并调节肠道菌群,从而缓解骨质疏松症。
老年人由于皮肤、肝肾功能的恶化,容易患骨质疏松症。补充维生素D (VD)的效果有限,VD缺乏主要是通过药物治疗。几项研究表明,肠道微生物群改变肠道VD代谢,益生菌补充剂可以影响循环VD水平。因此,在本研究中,我们通过模拟粪便细菌发酵,筛选了一株长双歧杆菌FSHHK13M1,该菌株可以提高体外发酵上清种中VD代谢物的水平。结果显示,FSHHK13M1干预显著提高血清1,25-二羟基VD和骨钙素水平。激活VDR、OPG、Wnt10b/β-catenin、Runx2/Osterix通路的表达,抑制RANKL/RANK通路的表达。骨小梁数量增加,骨体积比例增加。同时,骨质疏松小鼠的肠道微生物群表现出失衡的迹象。FSHHK13M1干预增加了特定细菌的相对丰度,如Faecalibaculum rodentium, Limosilactobacillus fermentum, pseudolongum双歧杆菌和Akkermansia muciniphila。上述结果提示,长叶假丝酵母FSHHK13M1通过调节相关基因、调节肠道菌群、提高活性VD水平,缓解维甲酸所致骨质疏松症症状。骨质疏松症是一种全身性代谢性疾病,患者由于各种原因导致骨量减少,骨组织微结构改变,导致骨脆性增加,易发生骨折。骨质疏松症在老年人中几乎是常见病,而跌倒骨折是老年人死亡风险的重要诱发因素。由于他们的肝脏、肾脏和皮肤功能下降,无法很好地利用维生素D,因此补充维生素D的效果非常有限。在目前的研究中,一种长双歧杆菌益生菌被发现可以增加维生素D活性形式的水平并改善骨质疏松症。这可能在预防骨质疏松症和降低老年人骨折风险方面发挥重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
mSphere
mSphere Immunology and Microbiology-Microbiology
CiteScore
8.50
自引率
2.10%
发文量
192
审稿时长
11 weeks
期刊介绍: mSphere™ is a multi-disciplinary open-access journal that will focus on rapid publication of fundamental contributions to our understanding of microbiology. Its scope will reflect the immense range of fields within the microbial sciences, creating new opportunities for researchers to share findings that are transforming our understanding of human health and disease, ecosystems, neuroscience, agriculture, energy production, climate change, evolution, biogeochemical cycling, and food and drug production. Submissions will be encouraged of all high-quality work that makes fundamental contributions to our understanding of microbiology. mSphere™ will provide streamlined decisions, while carrying on ASM''s tradition for rigorous peer review.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信