Prevalence and Phenotypic Characterization of Antibiotic-resistant Enterococcus Species Isolated from Chicken Faecal Samples in Accra, Ghana.

IF 1.5 Q3 HEALTH CARE SCIENCES & SERVICES
Philip Asumang, Frank Amoakohene, Collins Amponsah, Frank Kwasikumah, Emmanuel U Osisiogu, Israel M Attipoe
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Abstract

Background: Enterococci are bacteria found naturally in the gastrointestinal tract of both chickens and humans, serving as a commensal bacterium. These opportunistic pathogens are recognized for their involvement in human diseases like urinary tract infections (UTIs), endocarditis, and sepsis. Infection sources encompass food, hospital environments, and animals, particularly chickens. Their increasing resistance to multiple drugs poses a growing concern for public healthThis study aimed to isolate the enterococcus species and to determine their antibiotic susceptibility profiles.

Method: Swab samples of gut content from poultry in specific slaughterhouses located within selected markets in Accra were collected, cultured on MacConkey agar No.2, and incubated overnight for growth. Colonies suspected to be Enterococcus species were subjected to biochemical testing, and confirmed colonies underwent antibiotic testing against commonly used medications for bacterial infections. The Kirby-Bauer disk diffusion method was used to assess the antibiotic susceptibility of the recovered isolates.

Results: Among the 160 samples examined, 97 (61%) were found to be contaminated with Enterococcus species. Each of the isolates displayed resistance to a minimum of three antibiotic classes tested in the study. Notably, high levels of resistance were observed for specific antibiotics, including penicillin (91.75%), vancomycin (87.63%), and tetracycline (80.41).

Conclusion: The findings of this study revealed a high prevalence of multi-drug resistant Enterococcus species isolated from chicken rectal swab samples collected from three selected markets in Accra, Ghana. All the isolates exhibited resistance to at least three classes of antibiotics tested.

加纳阿克拉市鸡粪便中分离的耐抗生素肠球菌的流行和表型特征
背景:肠球菌是天然存在于鸡和人类胃肠道中的细菌,是一种共生细菌。这些机会性病原体被认为与尿路感染、心内膜炎和败血症等人类疾病有关。感染源包括食物、医院环境和动物,特别是鸡。肠球菌对多种药物的耐药性日益增加,引起了公众的关注。本研究旨在分离肠球菌并确定其抗生素敏感性。方法:从阿克拉选定市场的特定屠宰场收集家禽肠道内容物的拭子样本,在麦康基琼脂2号上培养,并孵育过夜以生长。对怀疑为肠球菌的菌落进行生化检测,对已确认的菌落进行抗生素检测,以对抗常用的细菌感染药物。采用Kirby-Bauer纸片扩散法对分离菌株进行药敏试验。结果:160份样品中,97份(61%)被肠球菌污染。每个分离株对研究中测试的至少三种抗生素类显示出耐药性。值得注意的是,对特定抗生素的耐药水平较高,包括青霉素(91.75%)、万古霉素(87.63%)和四环素(80.41%)。结论:本研究结果显示,从加纳阿克拉三个选定市场收集的鸡直肠棉签样本中分离出的多重耐药肠球菌非常普遍。所有分离株对至少三种抗生素均表现出耐药性。
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来源期刊
Ethiopian Journal of Health Sciences
Ethiopian Journal of Health Sciences HEALTH CARE SCIENCES & SERVICES-
CiteScore
2.10
自引率
8.30%
发文量
137
审稿时长
12 weeks
期刊介绍: Ethiopian Journal of Health Sciences is a general health science journal addressing clinical medicine, public health and biomedical sciences. Rarely, it covers veterinary medicine
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