A comprehensive analysis of antimicrobial resistance of clinical emm89 Streptococcus pyogenes in Japan.

IF 3.7 Q2 INFECTIOUS DISEASES

JAC-Antimicrobial Resistance Pub Date : 2025-02-19 eCollection Date: 2025-02-01 DOI:10.1093/jacamr/dlaf017

Weichen Gong, Masayuki Ono, Masaya Yamaguchi, Daisuke Motooka, Yujiro Hirose, Kotaro Higashi, Momoko Kobayashi, Eri Ikeda, Tomoko Sumitomo, Rumi Okuno, Takahiro Yamaguchi, Ryuji Kawahara, Hitoshi Otsuka, Noriko Nakanishi, Yu Kazawa, Chikara Nakagawa, Ryo Yamaguchi, Hiroo Sakai, Yuko Matsumoto, Tadayoshi Ikebe, Shigetada Kawabata

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Abstract

Objectives: Streptococcus pyogenes is involved in a wide range of diseases, including pharyngitis and life-threatening invasive infections. Increasing prevalence of antimicrobial resistance (AMR) has been reported worldwide in various bacteria, limiting the use of antibiotics in infection cases. The present study investigated the AMR of most prevalent S. pyogenes emm types, including emm89 strains in Japan.

Methods: A total of 368 previously identified S. pyogenes isolates (311 emm89 strains and 57 of other emm types), which were previously isolated from patients with invasive and non-invasive infections throughout Japan, were used in the analyses. The minimum inhibitory concentrations of seven antibiotics, including penicillin-G, azithromycin (AZM) and clindamycin, were determined, and whole-genome sequences of AMR-associated genes were screened.

Results: We identified 47 resistant strains, of which 91.49% (43/47) were resistant to AZM and/or clindamycin. A strong correlation was observed between non-invasive phenotypes and AMR. Whole-genome analysis indicated the wide distribution of three AMR-related genes, ermT, folP and lmrP, among the emm89 strains. Additionally, tetO was detected in tetracycline-resistance and soxS and mel was detected in chloramphenicol-resistance only in emm4 strains.

Conclusions: The high prevalence of S. pyogenes resistance to AZM and/or clindamycin poses a threat to public health in Japan; thus, the development of next-generation antimicrobial therapies is imperative.

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日本临床emm89化脓性链球菌耐药性综合分析

目的：化脓性链球菌与多种疾病有关，包括咽炎和危及生命的侵袭性感染。据报道，世界各地各种细菌的抗菌素耐药性（AMR）日益普遍，这限制了抗生素在感染病例中的使用。本研究调查了最流行的化脓性链球菌emm型的AMR，包括日本的emm89菌株。方法：采用从日本侵入性和非侵入性感染患者中分离到的368株既往鉴定的化脓性链球菌（311株emm89株和57株其他emm型）进行分析。测定青霉素-g、阿奇霉素（AZM）和克林霉素等7种抗生素的最低抑菌浓度，筛选抗菌素耐药性相关基因的全基因组序列。结果：共检出耐药菌株47株，其中对AZM和/或克林霉素耐药的占91.49%（43/47）。非侵入性表型与AMR之间存在很强的相关性。全基因组分析表明，emm89菌株中存在ermT、folP和lmrP 3个与amr相关的基因。此外，在四环素耐药菌株中检测到tetO，而在氯霉素耐药菌株中仅检测到soxS和mel。结论：日本化脓性链球菌对AZM和/或格林霉素的高耐药性对公共卫生构成威胁；因此，开发下一代抗菌疗法势在必行。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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