{"title":"Conformational Stability at Low Temperatures Using Single Protein Nanoaperture Optical Tweezers.","authors":"Keiran Letwin, Matthew Peters, Reuven Gordon","doi":"10.1021/acs.jpcb.4c07987","DOIUrl":null,"url":null,"abstract":"<p><p>Nanoaperture optical tweezers allow for trapping single proteins and detecting their conformational changes without modifying the protein, i.e., being free from labels or tethers. While past works have used laser heating as a way to vary the local temperature, this does not allow for probing of lower temperature values. Here we investigate the lower temperature dynamics of individual Bovine Serum Albumin (BSA) proteins with the help of a custom Peltier cooling stage. The BSA transitions between the normal (N) and fast (F) states. The normal form of BSA has a maximum occupancy at 21 ± 1 °C, which is interpreted as its maximum stability point for the compact N form with respect to the F form. In this way, it is possible to find the relative thermodynamic parameters of single proteins without requiring any modifications to the intrinsic structure.</p>","PeriodicalId":60,"journal":{"name":"The Journal of Physical Chemistry B","volume":" ","pages":""},"PeriodicalIF":2.8000,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Journal of Physical Chemistry B","FirstCategoryId":"1","ListUrlMain":"https://doi.org/10.1021/acs.jpcb.4c07987","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Nanoaperture optical tweezers allow for trapping single proteins and detecting their conformational changes without modifying the protein, i.e., being free from labels or tethers. While past works have used laser heating as a way to vary the local temperature, this does not allow for probing of lower temperature values. Here we investigate the lower temperature dynamics of individual Bovine Serum Albumin (BSA) proteins with the help of a custom Peltier cooling stage. The BSA transitions between the normal (N) and fast (F) states. The normal form of BSA has a maximum occupancy at 21 ± 1 °C, which is interpreted as its maximum stability point for the compact N form with respect to the F form. In this way, it is possible to find the relative thermodynamic parameters of single proteins without requiring any modifications to the intrinsic structure.
期刊介绍：
An essential criterion for acceptance of research articles in the journal is that they provide new physical insight. Please refer to the New Physical Insights virtual issue on what constitutes new physical insight. Manuscripts that are essentially reporting data or applications of data are, in general, not suitable for publication in JPC B.
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