Correction to Interferons Alpha, Beta, Gamma Each Inhibit HCV Replication at the Level of Internal Ribosome Entry Site-Mediated Translation

Abstract

Correction to Interferons Alpha, Beta, Gamma Each Inhibit Hepatitis C Virus Replication at the Level of Internal Ribosome Entry Site-mediated translation Liver International 25 (2005): 580–594.

Corrections to Figure 6

Figure 6 is deleted. Data in Figure 7 confirm the results that were previously shown in Figure 6. HCV IRES inhibition conclusion is also supported by data shown in Figure 5, Figure 10 and Figure 11. These corrections do not alter the conclusions of the manuscript.

Corrected text: (Page 586, left column)

High-level expression of the HCV core–GFP fusion protein was achieved in Huh-7 cells using a low amount of replication-defective adenovirus that expresses T7 RNA polymerase. This model allows us to examine cells expressing HCV directly under a fluorescent microscope without further immunological detection procedures. Expression of the HCV–GFP chimera in the presence of different concentrations of IFN-a2b after 24 h was examined.

Corrections to Figure 8

Figure 8 is deleted. Data shown in Figure 5 confirm the results that were previously shown in Figure 8. EMCV-IRES inhibition by IFN is also supported by data shown in Figure 10. These corrections do not alter the conclusions of the manuscript.

Corrected text (page 586–587)

Results presented in Figure 7 show that IFN-α, -β and -γ each inhibit the translation of the core-GFP fusion protein in a dose-dependent manner. The translation of HCV mRNA is mediated by an IRES mechanism, which is different from the translation of a capped message that occurs in eukaryotic cells. We have assessed the effects of IFNs using another positive-strand RNA virus that requires IRES for mRNA translation, the EMCV (Figure 5 and Figure 10).