Effects of lupeol on experimental testicular ischemiareperfusion damage in rats.

Abdurrahman Azzam, Ramazan Karabulut, Cem Kaya, Sibel Eryılmaz, Alparslan Kapisiz, Zafer Turkyilmaz, Mehmet Arda Inan, Gizem Yaz Aydin, Ali Atan, Kaan Sonmez
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Additionally, in the T1 and T2 groups, 100 mg/kg of lupeol was injected intraperitoneally 30 minutes before and immediately after detorsion. At the sixth hour,aBACKGROUND: Infertility and organ loss are potential consequences of testicular torsion, a urological emergency. This study aimed to evaluate the impact of lupeol on testicular ischemia-reperfusion damage.</p><p><strong>Methods: </strong>Thirty adult male Sprague-Dawley rats were randomly assigned to five groups: Control (C), Lupeol (L), Ischemia (Isc),Treatment 1 (T1), and Treatment 2 (T2). In the study groups, detorsion was applied to the left testicles following the induction of 720-degree testicular torsion for two hours. In the T1 and T2 groups, 100 mg/kg of lupeol was administered intraperitoneally 30 minutes before and immediately after detorsion. At the sixth hour, blood and testicular tissue samples were collected from each rat. Measurements included serum interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), tissue glutathione (GSH), malondialdehyde (MDA), and caspase-3 levels. Histopathological analysis was performed to assess the Johnsen Tubular Biopsy Score (JTBS).</p><p><strong>Results: </strong>Levels of caspase-3 (2.74+-0.32), MDA (1.71+-0.26), IL-6 (4.92+-0.57), and TNF-α (113.18+-29.77) were elevated in Group Isc compared to Group C and showed a significant reduction in Group T2 (2+-0.67, 1.16+-0.36, 3.95+-0.17, and 106.13+-12.49, respectively) and particularly in Group T1 (1.65+-0.50, 0.95+-0.143, 80+-0.35, and 104.86+-8.42, respectively) (p=0.001). However, while TNF-αlevels decreased in both treatment groups, the difference was not statistically significant (p=0.768). GSH levels decreased in Group Isc(140.63+-25.71) but increased in Group T2 (211.58+-95.05) (p=0.753) and particularly in Group T1 (219.9+-48.21)(p=0.078). The JTBS was lowest in Group Isc (7.67+-0.25). 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Abstract

Background: Infertility and organ loss are possible outcomes of testicular torsion, a urological emergency. This study aimed to demonstrate the impact of lupeol on testicular ischemia/reperfusion damage.

Methods: Thirty adult male Spraque-Dawley rats were randomized into five groups: Control (C), Lupeol (L), Ischemia (Isc), Treatment 1 (T1), and Treatment 2 (T2). In the study groups, detorsion was applied to the left testicles by creating a 720-degree testicular torsion for 2 h. Additionally, in the T1 and T2 groups, 100 mg/kg of lupeol was injected intraperitoneally 30 minutes before and immediately after detorsion. At the sixth hour,aBACKGROUND: Infertility and organ loss are potential consequences of testicular torsion, a urological emergency. This study aimed to evaluate the impact of lupeol on testicular ischemia-reperfusion damage.

Methods: Thirty adult male Sprague-Dawley rats were randomly assigned to five groups: Control (C), Lupeol (L), Ischemia (Isc),Treatment 1 (T1), and Treatment 2 (T2). In the study groups, detorsion was applied to the left testicles following the induction of 720-degree testicular torsion for two hours. In the T1 and T2 groups, 100 mg/kg of lupeol was administered intraperitoneally 30 minutes before and immediately after detorsion. At the sixth hour, blood and testicular tissue samples were collected from each rat. Measurements included serum interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), tissue glutathione (GSH), malondialdehyde (MDA), and caspase-3 levels. Histopathological analysis was performed to assess the Johnsen Tubular Biopsy Score (JTBS).

Results: Levels of caspase-3 (2.74+-0.32), MDA (1.71+-0.26), IL-6 (4.92+-0.57), and TNF-α (113.18+-29.77) were elevated in Group Isc compared to Group C and showed a significant reduction in Group T2 (2+-0.67, 1.16+-0.36, 3.95+-0.17, and 106.13+-12.49, respectively) and particularly in Group T1 (1.65+-0.50, 0.95+-0.143, 80+-0.35, and 104.86+-8.42, respectively) (p=0.001). However, while TNF-αlevels decreased in both treatment groups, the difference was not statistically significant (p=0.768). GSH levels decreased in Group Isc(140.63+-25.71) but increased in Group T2 (211.58+-95.05) (p=0.753) and particularly in Group T1 (219.9+-48.21)(p=0.078). The JTBS was lowest in Group Isc (7.67+-0.25). However, improvements were observed in both treatment groups (8.93+-0.16 and 8.82+-0.22, respectively) (p=0.001).

Conclusion: This study, the first to use lupeol in an experimental testicular torsion model, demonstrated its antioxidant, antiinflammatory, anti-apoptotic, histopathological damage-reducing, and protective effects. blood and testicular tissue samples were obtained from each rat. Serum interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), tissue glutathione (GSH), malondialdehyde (MDA), and caspase-3 measurements were also obtained. Histopathological analysis was used to evaluate the Johnsen Tubular Biopsy Score (JTBS).

Results: Caspase-3 (2,74+-0,32), MDA (1,71+-0,26), IL-6 (4,92+-0,57), and TNF-ɑ (113,18+-29,77) values increased in Group Isc compared to Group C and significantly decreased in T2 (2+-0,67, 1,16+-0,36, 3,95+-0,17, and 106,13+-12,49) and particularly T1 groups (1,65+-0,50, 0,95+-0,143, 80+-0,35, and 104,86+-8,42) (p=0.001). However, TNF-α levels decreased in both treatment groups, with no statistically significant difference (p=0.768). GSH levels decreased in Group Isc (140,63+-25,71) but increased in T2 (211,58+-95,05) (p=0.753) and particularly in T1 groups (219,9+-48,21) (p=0.078). JTBS was lowest in Group Isc (7,67+-0,25). Improvement was observed in both treatment groups (8,93+-0,16 and 8,82+-0,22) (p=0.001).

Conclusion: This study, which is the first to use lupeol in an experimental testicular torsion model, demonstrated its antioxidant, anti-inflammatory, antiapoptotic, and histopathological damage-reducing and protective effects.

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