Effects of lupeol on experimental testicular ischemiareperfusion damage in rats.

Abdurrahman Azzam, Ramazan Karabulut, Cem Kaya, Sibel Eryılmaz, Alparslan Kapisiz, Zafer Turkyilmaz, Mehmet Arda Inan, Gizem Yaz Aydin, Ali Atan, Kaan Sonmez
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Additionally, in the T1 and T2 groups, 100 mg/kg of lupeol was injected intraperitoneally 30 minutes before and immediately after detorsion. At the sixth hour,aBACKGROUND: Infertility and organ loss are potential consequences of testicular torsion, a urological emergency. This study aimed to evaluate the impact of lupeol on testicular ischemia-reperfusion damage.</p><p><strong>Methods: </strong>Thirty adult male Sprague-Dawley rats were randomly assigned to five groups: Control (C), Lupeol (L), Ischemia (Isc),Treatment 1 (T1), and Treatment 2 (T2). In the study groups, detorsion was applied to the left testicles following the induction of 720-degree testicular torsion for two hours. In the T1 and T2 groups, 100 mg/kg of lupeol was administered intraperitoneally 30 minutes before and immediately after detorsion. At the sixth hour, blood and testicular tissue samples were collected from each rat. Measurements included serum interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), tissue glutathione (GSH), malondialdehyde (MDA), and caspase-3 levels. Histopathological analysis was performed to assess the Johnsen Tubular Biopsy Score (JTBS).</p><p><strong>Results: </strong>Levels of caspase-3 (2.74+-0.32), MDA (1.71+-0.26), IL-6 (4.92+-0.57), and TNF-α (113.18+-29.77) were elevated in Group Isc compared to Group C and showed a significant reduction in Group T2 (2+-0.67, 1.16+-0.36, 3.95+-0.17, and 106.13+-12.49, respectively) and particularly in Group T1 (1.65+-0.50, 0.95+-0.143, 80+-0.35, and 104.86+-8.42, respectively) (p=0.001). However, while TNF-αlevels decreased in both treatment groups, the difference was not statistically significant (p=0.768). GSH levels decreased in Group Isc(140.63+-25.71) but increased in Group T2 (211.58+-95.05) (p=0.753) and particularly in Group T1 (219.9+-48.21)(p=0.078). The JTBS was lowest in Group Isc (7.67+-0.25). 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引用次数: 0

Abstract

Background: Infertility and organ loss are possible outcomes of testicular torsion, a urological emergency. This study aimed to demonstrate the impact of lupeol on testicular ischemia/reperfusion damage.

Methods: Thirty adult male Spraque-Dawley rats were randomized into five groups: Control (C), Lupeol (L), Ischemia (Isc), Treatment 1 (T1), and Treatment 2 (T2). In the study groups, detorsion was applied to the left testicles by creating a 720-degree testicular torsion for 2 h. Additionally, in the T1 and T2 groups, 100 mg/kg of lupeol was injected intraperitoneally 30 minutes before and immediately after detorsion. At the sixth hour,aBACKGROUND: Infertility and organ loss are potential consequences of testicular torsion, a urological emergency. This study aimed to evaluate the impact of lupeol on testicular ischemia-reperfusion damage.

Methods: Thirty adult male Sprague-Dawley rats were randomly assigned to five groups: Control (C), Lupeol (L), Ischemia (Isc),Treatment 1 (T1), and Treatment 2 (T2). In the study groups, detorsion was applied to the left testicles following the induction of 720-degree testicular torsion for two hours. In the T1 and T2 groups, 100 mg/kg of lupeol was administered intraperitoneally 30 minutes before and immediately after detorsion. At the sixth hour, blood and testicular tissue samples were collected from each rat. Measurements included serum interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), tissue glutathione (GSH), malondialdehyde (MDA), and caspase-3 levels. Histopathological analysis was performed to assess the Johnsen Tubular Biopsy Score (JTBS).

Results: Levels of caspase-3 (2.74+-0.32), MDA (1.71+-0.26), IL-6 (4.92+-0.57), and TNF-α (113.18+-29.77) were elevated in Group Isc compared to Group C and showed a significant reduction in Group T2 (2+-0.67, 1.16+-0.36, 3.95+-0.17, and 106.13+-12.49, respectively) and particularly in Group T1 (1.65+-0.50, 0.95+-0.143, 80+-0.35, and 104.86+-8.42, respectively) (p=0.001). However, while TNF-αlevels decreased in both treatment groups, the difference was not statistically significant (p=0.768). GSH levels decreased in Group Isc(140.63+-25.71) but increased in Group T2 (211.58+-95.05) (p=0.753) and particularly in Group T1 (219.9+-48.21)(p=0.078). The JTBS was lowest in Group Isc (7.67+-0.25). However, improvements were observed in both treatment groups (8.93+-0.16 and 8.82+-0.22, respectively) (p=0.001).

Conclusion: This study, the first to use lupeol in an experimental testicular torsion model, demonstrated its antioxidant, antiinflammatory, anti-apoptotic, histopathological damage-reducing, and protective effects. blood and testicular tissue samples were obtained from each rat. Serum interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), tissue glutathione (GSH), malondialdehyde (MDA), and caspase-3 measurements were also obtained. Histopathological analysis was used to evaluate the Johnsen Tubular Biopsy Score (JTBS).

Results: Caspase-3 (2,74+-0,32), MDA (1,71+-0,26), IL-6 (4,92+-0,57), and TNF-ɑ (113,18+-29,77) values increased in Group Isc compared to Group C and significantly decreased in T2 (2+-0,67, 1,16+-0,36, 3,95+-0,17, and 106,13+-12,49) and particularly T1 groups (1,65+-0,50, 0,95+-0,143, 80+-0,35, and 104,86+-8,42) (p=0.001). However, TNF-α levels decreased in both treatment groups, with no statistically significant difference (p=0.768). GSH levels decreased in Group Isc (140,63+-25,71) but increased in T2 (211,58+-95,05) (p=0.753) and particularly in T1 groups (219,9+-48,21) (p=0.078). JTBS was lowest in Group Isc (7,67+-0,25). Improvement was observed in both treatment groups (8,93+-0,16 and 8,82+-0,22) (p=0.001).

Conclusion: This study, which is the first to use lupeol in an experimental testicular torsion model, demonstrated its antioxidant, anti-inflammatory, antiapoptotic, and histopathological damage-reducing and protective effects.

芦皮酮对大鼠实验性睾丸缺血再灌注损伤的影响。
背景:睾丸扭转是泌尿外科急症,可能导致不孕和器官丧失。本研究旨在证明lupeople对睾丸缺血再灌注损伤的影响。方法:30只成年雄性Spraque-Dawley大鼠随机分为5组:对照组(C)、鲁皮醇组(L)、缺血组(Isc)、治疗1组(T1)、治疗2组(T2)。在研究组中,通过制造720度睾丸扭转2小时,对左侧睾丸施加扭转。此外,在T1和T2组中,在扭转前30分钟和扭转后立即腹腔注射100 mg/kg的lupeol。在第6小时,背景:不孕和器官丧失是睾丸扭转的潜在后果,泌尿外科急症。本研究旨在评价氟哌啶酮对睾丸缺血再灌注损伤的影响。方法:30只成年雄性Sprague-Dawley大鼠随机分为5组:对照组(C)、Lupeol组(L)、缺血组(Isc)、治疗1组(T1)、治疗2组(T2)。在研究组中,在诱导睾丸720度扭转2小时后,对左侧睾丸施加扭转。T1组和T2组分别于腐化前30分钟和腐化后立即腹腔注射卢皮酮100 mg/kg。在第6小时,从每只大鼠身上采集血液和睾丸组织样本。检测血清白介素-6 (IL-6)、肿瘤坏死因子-α (TNF-α)、组织谷胱甘肽(GSH)、丙二醛(MDA)和caspase-3水平。进行组织病理学分析以评估Johnsen肾小管活检评分(JTBS)。结果:与C组相比,Isc组caspase-3(2.74+-0.32)、MDA(1.71+-0.26)、IL-6(4.92+-0.57)、TNF-α(113.18+-29.77)水平升高,T2组显著降低(分别为2+-0.67、1.16+-0.36、3.95+-0.17、106.13+-12.49),尤其是T1组(1.65+-0.50、0.95+-0.143、80+-0.35、104.86+-8.42)(p=0.001)。两组患者TNF-α水平均有所下降,但差异无统计学意义(p=0.768)。Isc组GSH水平降低(140.63+-25.71),T2组升高(211.58+-95.05)(p=0.753), T1组升高(219.9+-48.21)(p=0.078)。JTBS在Isc组最低(7.67+-0.25)。然而,两个治疗组均有改善(分别为8.93+-0.16和8.82+-0.22)(p=0.001)。结论:本研究首次将芦皮醇应用于睾丸扭转实验模型,证明其具有抗氧化、抗炎、抗细胞凋亡、减轻组织病理学损伤和保护作用。取每只大鼠的血液和睾丸组织样本。测定血清白细胞介素-6 (IL-6)、肿瘤坏死因子-α (TNF-α)、组织谷胱甘肽(GSH)、丙二醛(MDA)和caspase-3。采用组织病理学分析评估Johnsen肾小管活检评分(JTBS)。结果:与C组相比,Isc组Caspase-3(2,74+-0,32)、MDA(1,71+-0,26)、IL-6(4,92+-0,57)、TNF- β(113、18+-29,77)值升高,T2组(2+-0,67、1,16+-0,36、3,95+-0,17和106、13+-12,49),尤其是T1组(1,65+-0,50、0,95+-0,143、80+-0,35和104、86+-8,42)显著降低(p=0.001)。两组患者TNF-α水平均下降,差异无统计学意义(p=0.768)。Isc组GSH水平降低(140,63+-25,71),T2组GSH水平升高(211,58+-95,05)(p=0.753),尤其是T1组(219,9+-48,21)(p=0.078)。JTBS在Isc组最低(7,67+-0,25)。两个治疗组均有改善(8,93+-0,16和8,82+-0,22)(p=0.001)。结论:本研究首次将芦皮醇应用于实验性睾丸扭转模型,表明其具有抗氧化、抗炎、抗细胞凋亡、减少组织病理学损伤和保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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