Retinal and metabolic changes in a high-fat diet (HFD)+STZ model of Type II diabetes.

IF 1.8 3区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Vision Pub Date : 2024-07-01 eCollection Date: 2024-01-01

Stephen Phillips, Andrew Feola, Jessica Solomon, Lidia Cardelle, Amber Douglass, Katie L Bales, Monica Coulter, Lauren Hutson, Cara T Khayat, Ally Grubman, Cody Worthy, Jeffrey H Boatright, Machelle T Pardue, Rachael S Allen

{"title":"Retinal and metabolic changes in a high-fat diet (HFD)+STZ model of Type II diabetes.","authors":"Stephen Phillips, Andrew Feola, Jessica Solomon, Lidia Cardelle, Amber Douglass, Katie L Bales, Monica Coulter, Lauren Hutson, Cara T Khayat, Ally Grubman, Cody Worthy, Jeffrey H Boatright, Machelle T Pardue, Rachael S Allen","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>While the high-dose streptozotocin (STZ; 100 mg/kg) rodent model is the gold standard in modeling Type I diabetes, models for Type II diabetes are needed for this more common form of diabetes. We investigated the retinal, cognitive, and metabolic alterations in a Type II diabetic model induced by high-fat diet (HFD) and low-dose STZ (30 mg/kg). Long Evans rats were assigned to naïve control, HFD, or HFD+STZ groups. Diabetic rats were further stratified into Type I and Type II based on metabolic assessments. Optomotor response (OMR, visual function), electroretinograms (retinal function), and Y-maze (cognitive function) were tested. Serum was analyzed for 12 metabolic markers using a multiplex panel. Type I rats showed severe increases in blood glucose accompanied by impairments in insulin and glucose tolerance, reduced bodyweight, and low insulin levels. In contrast, Type II rats showed moderate changes in blood glucose and insulin and glucose tolerance with weights and insulin levels similar to naïve controls. Type I and II rats showed OMR deficits (p<0.05) and electroretinogram changes (p<0.05). No cognitive deficits were observed. Type I rats displayed reduced serum levels of brain-derived neurotrophic factor (BDNF), C-Peptide, and leptin (p<0.05), and alterations in C-Peptide, PYY, and glucagon levels correlated with retinal function changes (p<0.05). Type II rats exhibited a moderate diabetic state while still developing retinal and visual deficits, which recapitulates phenotypes reported in patients.</p>","PeriodicalId":18866,"journal":{"name":"Molecular Vision","volume":"30 ","pages":"239-259"},"PeriodicalIF":1.8000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829795/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Vision","FirstCategoryId":"3","ListUrlMain":"","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

While the high-dose streptozotocin (STZ; 100 mg/kg) rodent model is the gold standard in modeling Type I diabetes, models for Type II diabetes are needed for this more common form of diabetes. We investigated the retinal, cognitive, and metabolic alterations in a Type II diabetic model induced by high-fat diet (HFD) and low-dose STZ (30 mg/kg). Long Evans rats were assigned to naïve control, HFD, or HFD+STZ groups. Diabetic rats were further stratified into Type I and Type II based on metabolic assessments. Optomotor response (OMR, visual function), electroretinograms (retinal function), and Y-maze (cognitive function) were tested. Serum was analyzed for 12 metabolic markers using a multiplex panel. Type I rats showed severe increases in blood glucose accompanied by impairments in insulin and glucose tolerance, reduced bodyweight, and low insulin levels. In contrast, Type II rats showed moderate changes in blood glucose and insulin and glucose tolerance with weights and insulin levels similar to naïve controls. Type I and II rats showed OMR deficits (p<0.05) and electroretinogram changes (p<0.05). No cognitive deficits were observed. Type I rats displayed reduced serum levels of brain-derived neurotrophic factor (BDNF), C-Peptide, and leptin (p<0.05), and alterations in C-Peptide, PYY, and glucagon levels correlated with retinal function changes (p<0.05). Type II rats exhibited a moderate diabetic state while still developing retinal and visual deficits, which recapitulates phenotypes reported in patients.

本刊更多论文

虽然高剂量链脲佐菌素（STZ；100 毫克/千克）啮齿动物模型是 I 型糖尿病模型的黄金标准，但这种更常见的糖尿病还需要 II 型糖尿病模型。我们研究了高脂饮食（HFD）和低剂量 STZ（30 毫克/千克）诱导的 II 型糖尿病模型中视网膜、认知和代谢的改变。Long Evans大鼠被分配到天真对照组、HFD组或HFD+STZ组。根据代谢评估结果，将糖尿病大鼠进一步分为 I 型和 II 型。测试了视运动反应（OMR，视觉功能）、视网膜电图（视网膜功能）和Y-迷宫（认知功能）。使用多重面板对血清中的 12 种代谢标记物进行了分析。I 型大鼠的血糖严重升高，同时伴有胰岛素和葡萄糖耐量的损害、体重减轻和胰岛素水平低。相比之下，II 型大鼠的血糖、胰岛素和葡萄糖耐量变化适中，体重和胰岛素水平与天真对照组相似。I 型和 II 型大鼠显示出 OMR 缺陷（p

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Molecular Vision 生物-生化与分子生物学

CiteScore

4.40

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Molecular Vision is a peer-reviewed journal dedicated to the dissemination of research results in molecular biology, cell biology, and the genetics of the visual system (ocular and cortical). Molecular Vision publishes articles presenting original research that has not previously been published and comprehensive articles reviewing the current status of a particular field or topic. Submissions to Molecular Vision are subjected to rigorous peer review. Molecular Vision does NOT publish preprints. For authors, Molecular Vision provides a rapid means of communicating important results. Access to Molecular Vision is free and unrestricted, allowing the widest possible audience for your article. Digital publishing allows you to use color images freely (and without fees). Additionally, you may publish animations, sounds, or other supplementary information that clarifies or supports your article. Each of the authors of an article may also list an electronic mail address (which will be updated upon request) to give interested readers easy access to authors.