Intraoral treatment of persimmon tannin, a polyphenol extracted from persimmon, significantly ameliorates gingivitis, plaque and halitosis via directly influence the periodontal bacteria Porphyromonas gulae

IF 3 3区医学 Q2 PHARMACOLOGY & PHARMACY

Journal of pharmacological sciences Pub Date : 2025-02-10 DOI:10.1016/j.jphs.2025.02.001

Megu Toyooka , Mao Kaneki , Chiharu Ohira , Azusa Hachiya , Tomoki Fukuyama

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引用次数: 0

Abstract

Background

As periodontal disease (PD) is an irreversible disorder, preventive dentistry in human and veterinary medicine has become pertinent. This study focused on persimmon tannin (PT) and examined its bactericidal, anti-halitosis, and anti-inflammatory effects by focusing on Porphyromonas gulae (P. gulae).

Methods

The direct effects of PT on P. gulae were evaluated in vitro. Pro-inflammatory cytokines secretion induced by P. gulae in the macrophage cell lines were determined. A clinical study in dogs with P. gulae-associated PD was performed by one-month intraoral treatment with 0.1% PT-containing gel.

Results

PT exhibited a significant bactericidal effect to P. gulae. The biofilm formation and methyl mercaptan generated by P. gulae was significantly decreased by PT even after a short exposure period. P. gulae-induced proinflammatory cytokine production in macrophage cell lines was inhibited by PT treatment in a dose-dependent manner. In a clinical study of dogs, intraoral treatment with 0.1% PT did not significantly influence the gingivitis and plaque scores, however, the concentrations of hydrogen sulfide and methyl mercaptan were also significantly decreased by the PT treatment. Although there was no anti-bacterial in vitro, P. gulae activity and DNA detection decreased with PT treatment.

Conclusions

These findings suggest that intraoral administration of PT can prevent PD.

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来源期刊

Journal of pharmacological sciences 医学-药学

CiteScore

6.20

自引率

2.90%

发文量

104

审稿时长

31 days

期刊介绍： Journal of Pharmacological Sciences (JPS) is an international open access journal intended for the advancement of pharmacological sciences in the world. The Journal welcomes submissions in all fields of experimental and clinical pharmacology, including neuroscience, and biochemical, cellular, and molecular pharmacology for publication as Reviews, Full Papers or Short Communications. Short Communications are short research article intended to provide novel and exciting pharmacological findings. Manuscripts concerning descriptive case reports, pharmacokinetic and pharmacodynamic studies without pharmacological mechanism and dose-response determinations are not acceptable and will be rejected without peer review. The ethnopharmacological studies are also out of the scope of this journal. Furthermore, JPS does not publish work on the actions of biological extracts unknown chemical composition.