Characterization of diet-linked amino acid pool influence on Fusobacterium spp. growth and metabolism.

IF 3.7 2区 生物学 Q2 MICROBIOLOGY
mSphere Pub Date : 2025-03-25 Epub Date: 2025-02-13 DOI:10.1128/msphere.00789-24
Avery V Robinson, Sarah J Vancuren, Massimo Marcone, Emma Allen-Vercoe
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引用次数: 0

Abstract

The genus Fusobacterium contains multiple proteolytic opportunistic pathogens that have been increasingly linked to colorectal cancer (CRC). "Oncomicrobes" such as these fusobacterial species within the gut microbiota may contribute to CRC onset and/or progression. Protein-rich diets may both directly increase CRC risk and enrich for proteolytic oncomicrobes, including Fusobacterium spp. Individual food substrates vary in amino acid content, and released amino acid content that is not absorbed in the small intestine may influence the growth of colonic proteolytic fermenters. Fusobacteria such as Fusobacterium spp. are known to preferentially metabolize certain amino acids. As such, some foods may better support the growth of these species within the colonic environment than others. To explore this, in this study, we created free amino acid pools (FAAPs) to represent proportions of amino acids in major proteins of three common dietary protein sources (soy, beef, and bovine milk). Growth curves were generated for 39 Fusobacterium spp. strains cultured in a dilute medium supplemented with each of the three FAAPs. Thereafter, amino acid use by 31 of the 39 Fusobacterium spp. strains in each FAAP treatment was assessed. FAAP supplementation increased growth metrics of all Fusobacterium spp. strains tested; however, the strains varied greatly in terms of the FAAP(s) generating the greatest increase in growth. Furthermore, the amino acid utilization strategy was highly variable between strains of Fusobacterium spp. Neither growth metrics nor amino acid utilization could be explained by species classification of Fusobacterium spp. strains. This report expands upon the previous knowledge of fusobacterial amino acid metabolism and indicates that proteolytic oncomicrobial activity should be assessed in the context of available protein sources.IMPORTANCEFusobacterium spp. including F. animalis, F. nucleatum, F. vincentii, and F. polymorphum are common oral commensals with emerging importance in diseases across multiple body sites, including CRC. CRC lesions associated with fusobacteria tend to result in poorer prognosis and increased disease recurrence. While Fusobacterium spp. are thought to colonize after tumorigenesis, little is known about the factors that facilitate this colonization. Protein-rich diets yielding readily metabolized free amino acids within the colon may promote the growth of proteolytic fermenters such as fusobacteria. Here, we show that variable concentrations of free amino acids within pools that represent different dietary protein sources differentially influence fusobacterial growth, including CRC-relevant strains of Fusobacterium spp. This work highlights the high degree of variation in fusobacterial amino acid utilization patterns and suggests differing proportions of dietary amino acids that reach the colon could influence fusobacterial growth.

日粮氨基酸库对梭杆菌生长和代谢影响的研究。
梭杆菌属含有多种蛋白水解条件致病菌,这些致病菌与结直肠癌(CRC)的关系日益密切。“肿瘤微生物”,如肠道菌群中的梭菌,可能导致结直肠癌的发生和/或进展。富含蛋白质的饮食可能会直接增加结直肠癌的风险,也会增加包括梭杆菌在内的蛋白水解性肿瘤微生物。单个食物底物的氨基酸含量不同,未被小肠吸收的氨基酸释放含量可能会影响结肠蛋白水解发酵剂的生长。众所周知,梭杆菌属等梭杆菌会优先代谢某些氨基酸。因此,一些食物可能比其他食物更能支持这些物种在结肠环境中的生长。为了探索这一点,在本研究中,我们创建了游离氨基酸池(FAAPs)来表示三种常见膳食蛋白质来源(大豆、牛肉和牛奶)中主要蛋白质中氨基酸的比例。在添加三种faap的稀培养基中培养39株梭杆菌,得到生长曲线。随后,对每种FAAP处理下39株梭杆菌中31株的氨基酸使用情况进行了评估。补充FAAP可提高所有梭杆菌菌株的生长指标;然而,在FAAP(s)方面,菌株差异很大,产生最大的增长。此外,菌株间氨基酸利用策略差异很大,无论是生长指标还是氨基酸利用都不能用菌株的种类分类来解释。该报告扩展了以前对梭杆菌氨基酸代谢的了解,并指出应在可用蛋白质来源的背景下评估蛋白质水解肿瘤微生物活性。包括动物梭菌、具核梭菌、文森梭菌和多形梭菌在内的梭菌属是常见的口腔共生菌,在包括CRC在内的多部位疾病中具有越来越重要的作用。与梭杆菌相关的结直肠癌病变往往导致预后较差和疾病复发率增加。虽然梭杆菌被认为在肿瘤发生后定植,但对促进这种定植的因素知之甚少。富含蛋白质的饮食在结肠内产生易于代谢的游离氨基酸,可促进蛋白质水解发酵菌(如梭杆菌)的生长。本研究表明,不同浓度的游离氨基酸对Fusobacterium spp的生长有不同的影响,包括与crc相关的Fusobacterium spp菌株。这项工作强调了Fusobacterium氨基酸利用模式的高度差异,并表明到达结肠的不同比例的膳食氨基酸可能影响Fusobacterium的生长。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
mSphere
mSphere Immunology and Microbiology-Microbiology
CiteScore
8.50
自引率
2.10%
发文量
192
审稿时长
11 weeks
期刊介绍: mSphere™ is a multi-disciplinary open-access journal that will focus on rapid publication of fundamental contributions to our understanding of microbiology. Its scope will reflect the immense range of fields within the microbial sciences, creating new opportunities for researchers to share findings that are transforming our understanding of human health and disease, ecosystems, neuroscience, agriculture, energy production, climate change, evolution, biogeochemical cycling, and food and drug production. Submissions will be encouraged of all high-quality work that makes fundamental contributions to our understanding of microbiology. mSphere™ will provide streamlined decisions, while carrying on ASM''s tradition for rigorous peer review.
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