Abundance of clinically relevant antimicrobial resistance genes in the golden jackal (Canis aureus) gut.

IF 3.7 2区 生物学 Q2 MICROBIOLOGY
mSphere Pub Date : 2025-03-25 Epub Date: 2025-02-13 DOI:10.1128/msphere.00819-24
Roi Lapid, Yair Motro, Hillary Craddock, Ikram Salah, Roni King, Katherine Winner, Gila Kahila Bar-Gal, Jacob Moran-Gilad
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引用次数: 0

Abstract

The spread of antimicrobial resistance (AMR) is a critical One Health issue. Wildlife could act as reservoirs or vehicles of AMR bacteria (ARBs) and AMR genes (ARGs) but are relatively understudied. We sought to investigate clinically relevant ARGs in golden jackals (Canis aureus) thriving near human settlements in Israel. Fecal samples were collected from 111 jackals across four regions over a 10-month period. Various animal and spatio-temporal metadata were collected. Samples were analyzed by quantitative PCR (qPCR) for beta-lactamases (blaTEM, blaCTX-M15, and blaSHV), qnrS and int1. A subset of samples was subject to shotgun metagenomic sequencing followed by resistome and microbiome analyses. qPCR detected a high prevalence of ARGs, including beta-lactamases (blaTEM-1, 96.4%; blaCTX-M-15, 51.4%, blaSHV, 15.3%), fluoroquinolone resistance (qnrS, 87.4%), and class 1 integrons (Int1, 94.6%). The blaTEM-1 gene was found to be more prevalent in adult jackals compared to younger ones. Metagenomic analysis of a subset of samples revealed a diverse gut microbiome harboring a rich resistome with tetracycline resistance genes being the most prevalent. Metagenome-assembled genome analysis further identified several ARGs associated with clinically relevant bacteria. These findings highlight the potential role of golden jackals as reservoirs for AMR and emphasize the need for ongoing surveillance to better understand AMR transmission dynamics at the wildlife-human interface.

Importance: The research highlights the potential role of the golden jackals as reservoirs for antimicrobial resistance (AMR). The high prevalence of clinically relevant AMR genes in these jackals emphasizes the need for ongoing surveillance and monitoring to better understand AMR transmission dynamics at the wildlife-human interface.

金豺(Canis aureus)肠道中临床相关抗菌耐药基因的丰度。
抗菌素耐药性(AMR)的传播是一个关键的健康问题。野生动物可能是抗菌素耐药性细菌(ARBs)和抗菌素耐药性基因(ARGs)的宿主或载体,但对它们的研究相对较少。我们试图调查在以色列人类住区附近繁殖的金豺(Canis aureus)的临床相关ARGs。在10个月的时间里,从四个地区的111只豺狼身上收集了粪便样本。收集了各种动物和时空元数据。采用定量PCR (qPCR)对样品进行β -内酰胺酶(blaTEM、blaCTX-M15和blaSHV)、qnrS和int1的检测。一部分样本进行散弹枪宏基因组测序,然后进行抵抗组和微生物组分析。qPCR检测到ARGs的高患病率,包括β -内酰胺酶(blaem -1, 96.4%;blaCTX-M-15, 51.4%, blaSHV, 15.3%),氟喹诺酮类耐药(qnrS, 87.4%)和1类整合子(Int1, 94.6%)。研究发现,与年轻的豺狼相比,成年豺狼的blatem1基因更为普遍。对样本子集的宏基因组分析显示,多种肠道微生物组含有丰富的抵抗组,其中四环素耐药基因最为普遍。宏基因组组装基因组分析进一步确定了几种与临床相关细菌相关的ARGs。这些发现强调了金豺作为AMR储存库的潜在作用,并强调了持续监测的必要性,以更好地了解野生动物-人类界面的AMR传播动态。重要性:该研究强调了金豺作为抗菌素耐药性(AMR)储存库的潜在作用。临床相关抗菌素耐药性基因在这些豺中的高流行率强调了持续监测和监测的必要性,以更好地了解野生动物-人类界面的抗菌素耐药性传播动态。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
mSphere
mSphere Immunology and Microbiology-Microbiology
CiteScore
8.50
自引率
2.10%
发文量
192
审稿时长
11 weeks
期刊介绍: mSphere™ is a multi-disciplinary open-access journal that will focus on rapid publication of fundamental contributions to our understanding of microbiology. Its scope will reflect the immense range of fields within the microbial sciences, creating new opportunities for researchers to share findings that are transforming our understanding of human health and disease, ecosystems, neuroscience, agriculture, energy production, climate change, evolution, biogeochemical cycling, and food and drug production. Submissions will be encouraged of all high-quality work that makes fundamental contributions to our understanding of microbiology. mSphere™ will provide streamlined decisions, while carrying on ASM''s tradition for rigorous peer review.
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