Mechanism of Astragaloside IV in Promoting Osteogenic Differentiation.

IF 2.2 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Current molecular medicine Pub Date : 2025-02-12 DOI:10.2174/0115665240333454241203050356

Yao Zhu, Chengyu Lin, Xudong Zhang, Zuyun Qiu, Lei Shi, Jianmin Li

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引用次数: 0

Abstract

Background: This study focuses on exploring the impact of Astragaloside IV [AS-IV] on osteogenic differentiation.

Methods: Osteogenic differentiation was induced in rat osteoblasts, following which treatment with AS-IV at varied doses was performed. Using Alizarin red staining and alkaline phosphatase [ALP] detection assay, the osteogenic differentiation of the cells was investigated. The expressions of osteogenic differentiation-related genes were determined by quantitative real-time polymerase chain reaction [qRT-PCR]. The phosphatidylinositol 3-kinase [PI3K]/protein kinase B [Akt] signaling pathwayassociated protein expressions were examined using Western blot. After osteoblasts were transfected with protein tyrosine phosphatase non-receptor type 2 [PTPN2] overexpression plasmid, the impact of PTPN2 on osteoblasts treated with AS-IV was examined.

Results: AS-IV treatment enhanced osteogenic differentiation and up-regulated the expression of osteogenic differentiation-related genes, as well as the levels of p- PI3K/PI3K and p-AKT/AKT, while reducing PTEN protein production in osteoblasts. Overexpression of phosphatase and tensin homolog [PTEN] inhibited osteogenic differentiation, and PTPN2 overexpression counteracted the effects of AS-IV on osteogenic differentiation.

Conclusion: AS-IV contributing to osteogenic differentiation may be related to the PTPN2-mediated PTEN/PI3K/Akt pathway.

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黄芪甲苷促进成骨分化的机制。

背景：本研究主要探讨黄芪甲苷[AS-IV]对成骨分化的影响。方法：诱导大鼠成骨细胞成骨分化，并用不同剂量的AS-IV处理成骨细胞。采用茜素红染色和碱性磷酸酶（ALP）检测，观察细胞的成骨分化情况。采用实时定量聚合酶链反应（qRT-PCR）检测成骨分化相关基因的表达。Western blot检测磷脂酰肌醇3-激酶(PI3K) /蛋白激酶B （Akt）信号通路相关蛋白的表达。将蛋白酪氨酸磷酸酶非受体2型[PTPN2]过表达质粒转染成骨细胞后，检测PTPN2对AS-IV处理成骨细胞的影响。结果：as - iv处理增强成骨分化，上调成骨分化相关基因的表达以及p- PI3K/PI3K和p-AKT/AKT水平，同时减少成骨细胞PTEN蛋白的产生。磷酸酶和紧张素同源物PTEN过表达抑制成骨分化，PTPN2过表达抵消AS-IV对成骨分化的影响。结论：AS-IV参与成骨分化可能与ptpn2介导的PTEN/PI3K/Akt通路有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current molecular medicine 医学-医学：研究与实验

CiteScore

5.00

自引率

4.00%

发文量

141

审稿时长

4-8 weeks

期刊介绍： Current Molecular Medicine is an interdisciplinary journal focused on providing the readership with current and comprehensive reviews/ mini-reviews, original research articles, short communications/letters and drug clinical trial studies on fundamental molecular mechanisms of disease pathogenesis, the development of molecular-diagnosis and/or novel approaches to rational treatment. The reviews should be of significant interest to basic researchers and clinical investigators in molecular medicine. Periodically the journal invites guest editors to devote an issue on a basic research area that shows promise to advance our understanding of the molecular mechanism(s) of a disease or has potential for clinical applications.