Biodistribution of 131I-labelled monoclonal antibodies in human colon tumours by an ex vivo perfusion model.

International journal of cancer. Supplement = Journal international du cancer. Supplement Pub Date : 1988-01-01

E Kraas, E Löhde, O Abri, H Schlicker, S Matzku, H Kalthoff, W Schmiegel, R Arndt

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Abstract

We describe a model for the evaluation of anti-tumour antibody specificity, using a human carcinoma-bearing colon segment. After resection of the human colon tumour, the supplying artery was cannulated and perfused with fresh frozen plasma and heparin. Continuous control of pressure, flow, temperature, pH and various metabolic parameters were performed after administration of 131I-labelled anti-CEA antibody. Highly differentiated adenocarcinomas of the colon showed a much higher antibody uptake than undifferentiated tumours. Between 3 and 7% of the injected antibody was found in the tumour tissue. Autoradiography showed non-homogeneous binding in the tumour tissue. The non-specific antibody perfusion showed no tumour binding. We conclude that the ex vivo perfusion of resected colon carcinomas can be used to measure the kinetics of binding and clearance of MAbs in tumour tissue by direct scintigraphy. The cellular biodistribution of the antibody can be documented by means of autoradiography.

本刊更多论文

131 - i标记单克隆抗体在人结肠肿瘤的体外灌注模型中的生物分布。

我们描述了一个模型，用于评估抗肿瘤抗体特异性，使用人类结肠癌段。人结肠肿瘤切除后，给供血动脉插管，灌注新鲜冷冻血浆和肝素。在给予131i标记的抗cea抗体后，连续控制压力、流量、温度、pH和各种代谢参数。高度分化的结肠腺癌显示出比未分化的肿瘤更高的抗体摄取。在肿瘤组织中发现了注射抗体的3%到7%。放射自显影显示肿瘤组织的结合不均匀。非特异性抗体灌注未见肿瘤结合。我们得出结论，切除结肠癌的体外灌注可以通过直接闪烁成像来测量肿瘤组织中单克隆抗体的结合和清除动力学。抗体的细胞生物分布可以用放射自显影法记录。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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International journal of cancer. Supplement = Journal international du cancer. Supplement

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