Highly specific immunoassays for hazardous phenacetin in herbal teas enabled by low electronegativity-induced coating antigen

IF 3.2 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Food Science Pub Date : 2025-02-14 DOI:10.1111/1750-3841.70043

Maner Chen, Wei Lai, Jianhao Lin, Zhiwei Liu, Yi Lei, Yu Wang, Chenglong Wang, Xiaoqin Yu, Qiuhua Mo, Bingzhi Wang, Hongtao Lei, Tian Guan

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引用次数: 0

Abstract

Phenacetin is often illegally adulterated into herbal teas for enhanced analgesic and anti-inflammatory properties, posing great threats to consumers’ health. However, few rapid tests are available to monitor the banned phenacetin. Though one phenacetin immunoassay has been reported already, its unmet selectivity limits the wider application in rapid screening. Herein, a highly specific and sensitive phenacetin antibody was induced by long spacer arms-based immunogens coupling with low-electronegativity coating antigen. Furthermore, a precise indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a field practical colloidal gold immunochromatographic assay (AuNPs-LFIA) were developed for phenacetin detection in Guangdong herbal teas. This work exhibits low detection limits (8.80 ng/mL for ic-ELISA, 2.19 ng/mL for AuNPs-LFIA) and reasonable recoveries (102.8%–111.1% and 91.68%–111.91%). It is noteworthy that both of the developed ic-ELISA and AuNPs-LFIA have high accuracy and practicability in blind herbal tea analysis, which was validated by the authorized liquid chromatography–mass spectrometry/mass spectrometry method. The proposed ic-ELISA and AuNPs-LFIA could be promising approaches in monitoring Guangdong herbal teas with potential illegally adulterated phenacetin.

Practical Application

Rapid and accurate monitoring of illicitly added drugs of phenacetin in herbal teas is necessary and important for consumers and regulatory agencies.

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来源期刊

Journal of Food Science 工程技术-食品科技

CiteScore

7.10

自引率

2.60%

发文量

412

审稿时长

3.1 months

期刊介绍： The goal of the Journal of Food Science is to offer scientists, researchers, and other food professionals the opportunity to share knowledge of scientific advancements in the myriad disciplines affecting their work, through a respected peer-reviewed publication. The Journal of Food Science serves as an international forum for vital research and developments in food science. The range of topics covered in the journal include: -Concise Reviews and Hypotheses in Food Science -New Horizons in Food Research -Integrated Food Science -Food Chemistry -Food Engineering, Materials Science, and Nanotechnology -Food Microbiology and Safety -Sensory and Consumer Sciences -Health, Nutrition, and Food -Toxicology and Chemical Food Safety The Journal of Food Science publishes peer-reviewed articles that cover all aspects of food science, including safety and nutrition. Reviews should be 15 to 50 typewritten pages (including tables, figures, and references), should provide in-depth coverage of a narrowly defined topic, and should embody careful evaluation (weaknesses, strengths, explanation of discrepancies in results among similar studies) of all pertinent studies, so that insightful interpretations and conclusions can be presented. Hypothesis papers are especially appropriate in pioneering areas of research or important areas that are afflicted by scientific controversy.