The non-monotonic effect of sucrose on interactions between lipid-bearing surfaces

IF 9.4 1区 化学 Q1 CHEMISTRY, PHYSICAL
Yihui Dong , Yaelle Schilt , Roman Kamyshinsky , Nir Kampf , Qirong Zhu , Di Jin , Sharon Grayer Wolf , Uri Raviv , Jacob Klein
{"title":"The non-monotonic effect of sucrose on interactions between lipid-bearing surfaces","authors":"Yihui Dong ,&nbsp;Yaelle Schilt ,&nbsp;Roman Kamyshinsky ,&nbsp;Nir Kampf ,&nbsp;Qirong Zhu ,&nbsp;Di Jin ,&nbsp;Sharon Grayer Wolf ,&nbsp;Uri Raviv ,&nbsp;Jacob Klein","doi":"10.1016/j.jcis.2025.02.044","DOIUrl":null,"url":null,"abstract":"<div><div>The extremely low sliding friction of articular cartilage in synovial joints has been attributed to phospholipid boundary layers, lubricating via the hydration lubrication mechanism at their exposed, highly hydrated polar-head-groups, in a medium – the synovial fluid – where osmolytes, which may modify the hydration layer, are ubiquitous. Here, using a surface force balance (SFB), we carried out a systematic study to elucidate the effect of sucrose, a known osmotic regulator solute, with concentrations <em>c<sub>sucrose</sub></em>, ranging from 5 to 20 wt%, on the normal and shear forces between interacting phosphatidylcholine (PC) bilayers, both in the gel (1,2-dipalmitoyl-<em>sn</em>-<em>glycero</em>-3-phosphocholine, DPPC) and liquid (1,2-dimyristoyl-<em>sn</em>-<em>glycero</em>-3-phosphocholine, DMPC) phases, supported on atomically-smooth mica substrates. Several additional approaches including cryo-transmission electron microscope, atomic force microscopy, small- and wide-angle X-ray scattering, differential scanning calorimetry, dynamic light scattering and zeta potential measurements are exploited to get additional insight into the nature of the sucrose-dependent interactions. As <em>c<sub>sucrose</sub></em> is varied, a remarkable variation in the friction is observed: a marked <em>reduction</em> in friction is seen at low <em>c<sub>sucrose</sub></em>, but at higher sucrose levels the friction increases, for both gel and liquid phase lipids. This challenges the expectation that hydration lubrication is degraded by osmotic solutes, due to their competing for water of hydration, and reveals for the first time a <em>non-monotonic</em> effect of a sugar on the interactions, particularly frictional forces, between lipid bilayers. This non-monotonic effect correlates with the bilayer potential, and is attributed to a concentration-dependent affinity of the sugar to the PC headgroups.</div></div>","PeriodicalId":351,"journal":{"name":"Journal of Colloid and Interface Science","volume":"687 ","pages":"Pages 217-229"},"PeriodicalIF":9.4000,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Colloid and Interface Science","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0021979725003959","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
引用次数: 0

Abstract

The extremely low sliding friction of articular cartilage in synovial joints has been attributed to phospholipid boundary layers, lubricating via the hydration lubrication mechanism at their exposed, highly hydrated polar-head-groups, in a medium – the synovial fluid – where osmolytes, which may modify the hydration layer, are ubiquitous. Here, using a surface force balance (SFB), we carried out a systematic study to elucidate the effect of sucrose, a known osmotic regulator solute, with concentrations csucrose, ranging from 5 to 20 wt%, on the normal and shear forces between interacting phosphatidylcholine (PC) bilayers, both in the gel (1,2-dipalmitoyl-sn-glycero-3-phosphocholine, DPPC) and liquid (1,2-dimyristoyl-sn-glycero-3-phosphocholine, DMPC) phases, supported on atomically-smooth mica substrates. Several additional approaches including cryo-transmission electron microscope, atomic force microscopy, small- and wide-angle X-ray scattering, differential scanning calorimetry, dynamic light scattering and zeta potential measurements are exploited to get additional insight into the nature of the sucrose-dependent interactions. As csucrose is varied, a remarkable variation in the friction is observed: a marked reduction in friction is seen at low csucrose, but at higher sucrose levels the friction increases, for both gel and liquid phase lipids. This challenges the expectation that hydration lubrication is degraded by osmotic solutes, due to their competing for water of hydration, and reveals for the first time a non-monotonic effect of a sugar on the interactions, particularly frictional forces, between lipid bilayers. This non-monotonic effect correlates with the bilayer potential, and is attributed to a concentration-dependent affinity of the sugar to the PC headgroups.

Abstract Image

求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
16.10
自引率
7.10%
发文量
2568
审稿时长
2 months
期刊介绍: The Journal of Colloid and Interface Science publishes original research findings on the fundamental principles of colloid and interface science, as well as innovative applications in various fields. The criteria for publication include impact, quality, novelty, and originality. Emphasis: The journal emphasizes fundamental scientific innovation within the following categories: A.Colloidal Materials and Nanomaterials B.Soft Colloidal and Self-Assembly Systems C.Adsorption, Catalysis, and Electrochemistry D.Interfacial Processes, Capillarity, and Wetting E.Biomaterials and Nanomedicine F.Energy Conversion and Storage, and Environmental Technologies
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信