Satellitome analysis on Microtus thomasi (Arvicolinae) genome, a mammal species with high karyotype and sex chromosome variations.

IF 2.3 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Genome Pub Date : 2025-01-01 DOI:10.1139/gen-2024-0141
Gaël Aleix-Mata, Eugenia E Montiel, Pablo Mora, Alona Yurchenko, José M Rico-Porras, Francisco Anguita, Fátima Palomo, Juan Alberto Marchal, Michail Rovatsos, Antonio Sánchez
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引用次数: 0

Abstract

The voles of the Microtus thomasi/Microtus atticus species complex (Arvicolinae) display extensive karyotypic variation, in the number of autosomes and the morphology of sex chromosomes. We analyzed the satellitome of Microtus thomasi and identified 17 satellite DNA (satDNA) families, corresponding to 6.704% of the genome. Homogenization and divergence analyses showed that some satDNA families are more homogeneous, indicative of recent amplification, while others displayed higher variation, suggesting ancient amplification. Twelve of the satDNA families are conserved across Arvicolinae with a substantial variation in the abundance and the composition. These results support the "library" hypothesis, where a shared collection of satDNAs exists across related species, with differential amplification driving species-specific genomic profiles. Localization analysis demonstrated that an increased number of satDNA families are localized in the pericentromeric and the heterochromatic regions of autosomes and sex chromosomes. Our results suggest that the heterochromatin of the X and Y chromosomes co-evolved and that satDNA families might have contributed to the chromosomal rearrangements involved in the karyotypic variation and sex chromosome polymorphism of the chromosomal races. Our study contributes to a deeper understanding of the evolutionary mechanisms underlying karyotype diversification in Microtus species, which exhibit some of the highest rates of karyotypic variation among mammals.

对具有高度核型和性染色体变异的哺乳动物--Microtus thomasi(Arvicolinae)基因组进行卫星组分析。
田鼠群在常染色体数量和性染色体形态上表现出广泛的核型变异。我们分析了M. thomasi的卫星组,鉴定出17个卫星dna家族,对应基因组的6.704%。均质化和分化分析表明,一些卫星dna家族更均匀,表明最近的扩增,而另一些则表现出更高的变异,表明古代扩增。在Arvicolinae中有12个satDNA家族保守,在丰度和组成上存在较大差异。这些结果支持了“文库”假说,即在相关物种之间存在一个共享的satdna集合,通过差异扩增驱动物种特异性基因组图谱。定位分析表明,越来越多的satDNA家族定位于常染色体和性染色体的近中心和异色区。我们的研究结果表明,X和Y染色体的异染色质共同进化,并且satDNA家族可能参与了染色体重排,涉及染色体种族的核型变异和性染色体多态性。我们的研究有助于更深入地了解鼠种核型多样化的进化机制,鼠种在哺乳动物中表现出一些最高的核型变异率。
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来源期刊
Genome
Genome 生物-生物工程与应用微生物
CiteScore
5.30
自引率
3.20%
发文量
42
审稿时长
6-12 weeks
期刊介绍: Genome is a monthly journal, established in 1959, that publishes original research articles, reviews, mini-reviews, current opinions, and commentaries. Areas of interest include general genetics and genomics, cytogenetics, molecular and evolutionary genetics, developmental genetics, population genetics, phylogenomics, molecular identification, as well as emerging areas such as ecological, comparative, and functional genomics.
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