Dynamic Observation of Colonic Lesions, Commensal Microbiome, and Mycobiome Variations in Trinitrobenzene Sulfonic Acid-Induced Experimental Crohn's Disease in Rats.

IF 3.6 3区 医学 Q2 GASTROENTEROLOGY & HEPATOLOGY
Digestion Pub Date : 2025-02-11 DOI:10.1159/000543337
Mingjiang Liu, Huiwen Wang, Junjie Huang, Yuying Huai, Chenglong Yu, Guoqing Fang, Minxia Zhang, Ruonan Bo, Kai Fan, Jingui Li
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引用次数: 0

Abstract

Introduction: Crohn's disease (CD) is an inflammatory bowel disease characterized by chronic inflammation of the entire digestive lining. Although the pathogenesis of CD remains unclear, multiple factors, especially altered microbiota, are among its causes.

Methods: In this study, an experimental CD model was established by trinitrobenzene sulfonic acid (TNBS) enema. Then the dynamic changes of colonic tissue lesions, tight junctions, inflammation response, and oxidative stress are respectively tested by hematoxylin and eosin staining, immunofluorescence staining, and commercial kits. 16S rRNA and ITS sequencing of colonic feces were applied to analyze the composition and diversity of the microbiome and mycobiome for lasting 5 weeks.

Results: As a result, despite TNBS being applied only once time, the stimuli-caused injury reached a peak in the second week (the most severe period), after which symptoms began to gradually return to the normal stage. Additionally, consistent with the TNBS-caused colonic damage, deaths were also concentrated within 2 weeks after modeling, with only one death occurring in the subsequent period despite ongoing inflammation and other typical symptoms. In terms of gut bacteria, microbiome diversity decreased significantly while mycobiome diversity increased, along with the enrichment of harmful microbiota and shrinkage of probiotic microorganisms.

Conclusion: Therefore, the data suggested that TNBS-induced CD can be roughly divided into two phases: the acute inflammatory phase (weeks 1-2) and the chronic inflammatory phase (weeks 3-5). However, the microbiome and mycobiome dysbiosis did not return to normal within the trial period. Hence, our findings may facilitate a better comprehension of the dynamic progress of experimental TNBS-induced CD.

三硝基苯磺酸致实验性克罗恩病大鼠结肠病变、共生微生物组和真菌组变化的动态观察
克罗恩病(CD)是一种炎症性肠病,其特征是整个消化系统的慢性炎症。虽然乳糜泻的发病机制尚不清楚,但多种因素,特别是微生物群的改变是其原因之一。本研究采用tnbs灌肠法建立实验性CD模型。然后分别采用H&;E染色、免疫荧光染色和商用试剂盒检测结肠组织病变、紧密连接、炎症反应和氧化应激的动态变化。用16S rRNA和ITS测序法分析结肠粪便微生物组和真菌组的组成和多样性,持续5周。因此,尽管TNBS只应用一次,但刺激引起的损伤在第二周(最严重的时期)达到高峰,之后症状开始逐渐恢复到正常阶段。此外,与tnbs引起的结肠损伤一致,死亡也集中在建模后两周内,尽管持续存在炎症和其他典型症状,但在随后的一段时间内仅发生一例死亡。肠道菌群方面,微生物组多样性显著降低,而真菌组多样性显著增加,有害菌群富集,益生菌群萎缩。因此,数据提示tnbs诱导的CD大致可以分为急性炎症期(1 ~ 2周)和慢性炎症期(3 ~ 5周)两个阶段,但在试验期内,微生物组和真菌组的失调并没有恢复正常。因此,我们的研究结果可能有助于更好地理解实验性tnbs诱导CD的动态进展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Digestion
Digestion 医学-胃肠肝病学
CiteScore
7.90
自引率
0.00%
发文量
39
审稿时长
6-12 weeks
期刊介绍: ''Digestion'' concentrates on clinical research reports: in addition to editorials and reviews, the journal features sections on Stomach/Esophagus, Bowel, Neuro-Gastroenterology, Liver/Bile, Pancreas, Metabolism/Nutrition and Gastrointestinal Oncology. Papers cover physiology in humans, metabolic studies and clinical work on the etiology, diagnosis, and therapy of human diseases. It is thus especially cut out for gastroenterologists employed in hospitals and outpatient units. Moreover, the journal''s coverage of studies on the metabolism and effects of therapeutic drugs carries considerable value for clinicians and investigators beyond the immediate field of gastroenterology.
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