Generation of Retinal Organoids Using Human-Induced Pluripotent Stem Cells.

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-02-11 DOI:10.1007/7651_2024_600

Keith Theodore, Joel Alan Imventarza, Saleha Tahir, Bruna Lopes da Costa, Peter M J Quinn

引用次数: 0

Abstract

Retinal organoids (ROs) derived from human-induced pluripotent stem cells (hiPSCs) serve as relevant models for studying retinal disease pathogenesis, as well as furthering gene therapy efforts. These complex, three-dimensional (3D), multicellular structures recapitulate the development and functionality of the maturing human retina. Here, we describe an in-depth method for the generation of ROs from hiPSCs and evaluate the morphology of these multilayered structures.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.