Activation of CXCR3+ Tfh cells and B cells in lymph nodes during acute HIV-1 infection correlates with HIV-specific antibody development.

IF 4 2区 医学 Q2 VIROLOGY
Journal of Virology Pub Date : 2025-03-18 Epub Date: 2025-02-11 DOI:10.1128/jvi.01532-24
Julie L Mitchell, Supranee Buranapraditkun, Pierre Gantner, Hiroshi Takata, Kenneth Dietze, Kombo F N'guessan, Justin Pollara, Junsuke Nohara, Roshell Muir, Eugene Kroon, Suteeraporn Pinyakorn, Nicha Tulmethakaan, Sopark Manasnayakorn, Suthat Chottanapund, Pattarawat Thantiworasit, Peeriya Prueksakaew, Nisakorn Ratnaratorn, Suwanna Puttamaswin, Bessara Nuntapinit, Lawrence Fox, Elias K Haddad, Dominic Paquin-Proulx, Praphan Phanuphak, Carlo P Sacdalan, Nittaya Phanuphak, Jintanat Ananworanich, Denise Hsu, Sandhya Vasan, Guido Ferrari, Nicolas Chomont, Lydie Trautmann
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Abstract

Lymph node T follicular helper (Tfh) cells and germinal center (GC) B cells are critical to generate potent antibodies but are rarely possible to study in humans. To understand how Tfh/GC B-cell interactions during acute HIV-1 infection (AHI) impact the generation of HIV-specific antibodies, we performed a unique cross-sectional analysis of inguinal lymph node biopsies taken prior to antiretroviral therapy (ART) initiation in AHI. Although total Tfh and GC B cell frequencies did not change during AHI, increased frequencies of proliferating Th1-like CXCR3+ Tfh, CXCR3+ non-GC B cells, and total CXCR3+ GC B cells correlated with gp120-specific IgG antibody levels in AHI. Frequencies of proliferating CXCR3+ Tfh in AHI also correlated with gp120-specific IgG antibody levels after 48 weeks of ART, antibody-dependent cellular cytotoxicity, antibody-dependent cellular phagocytosis, and increased antibody binding to infected cells after ART. Importantly, while beneficial for antibody development, CXCR3+ Tfh cells were also infected by HIV-1 at higher frequencies than their CXCR3- counterparts and may contribute to the initial dissemination of HIV-1 in follicles. Together, these data suggest that activation of CXCR3+ Tfh cells is associated with induction of the germinal center response and subsequent antibody development, making these cells an important target for future therapeutic interventions.

Importance: Early initiation of antiretroviral therapy (ART) is important to limit the seeding of the long-lasting HIV-1 reservoir; however, it also precludes the development of HIV-specific antibodies that can help control the virus if ART is stopped. Antibody development occurs within germinal centers in the lymph node and requires activation of both antigen-specific B cells and T follicular helper cells (Tfh), a specialized CD4+ cell that provides B cell help. To understand how early ART initiation may prohibit antibody development, we analyzed the frequencies and activation status of Tfh and B cells in lymph node biopsies collected in the different stages of acute HIV-1 infection. Our data suggest that decreased antibody development after early ART initiation may be due to limited germinal center development at the time of treatment and that new interventions that target activation of CXCR3+ Tfh may be beneficial to increase long-term HIV-specific antibody levels.

急性HIV-1感染期间淋巴结中CXCR3+ Tfh细胞和B细胞的激活与hiv特异性抗体的产生相关。
淋巴结T滤泡辅助细胞(Tfh)和生发中心(GC) B细胞是产生强效抗体的关键细胞,但很少有可能在人体中进行研究。为了了解急性HIV-1感染(AHI)期间Tfh/GC b细胞相互作用如何影响hiv特异性抗体的产生,我们对AHI患者开始抗逆转录病毒治疗(ART)之前进行的腹股沟淋巴结活检进行了独特的横断面分析。虽然在AHI期间总Tfh和GC B细胞频率没有变化,但th1样CXCR3+ Tfh、CXCR3+非GC B细胞和总CXCR3+ GC B细胞的增殖频率增加与AHI中gp120特异性IgG抗体水平相关。在抗逆转录病毒治疗48周后,AHI中增殖CXCR3+ Tfh的频率也与gp120特异性IgG抗体水平、抗体依赖性细胞毒性、抗体依赖性细胞吞噬以及抗逆转录病毒治疗后感染细胞的抗体结合增加相关。重要的是,虽然有利于抗体的产生,但CXCR3+ Tfh细胞也以比CXCR3-细胞更高的频率被HIV-1感染,并可能有助于HIV-1在卵泡中的初始传播。总之,这些数据表明,CXCR3+ Tfh细胞的激活与生发中心反应的诱导和随后的抗体产生有关,使这些细胞成为未来治疗干预的重要靶点。重要性:早期开始抗逆转录病毒治疗(ART)对于限制长期存在的HIV-1病毒库的播种非常重要;然而,如果停止抗逆转录病毒治疗,它也阻碍了艾滋病毒特异性抗体的产生,这些抗体可以帮助控制病毒。抗体的产生发生在淋巴结的生发中心,需要抗原特异性B细胞和T滤泡辅助细胞(Tfh)的激活,Tfh是一种专门提供B细胞帮助的CD4+细胞。为了了解早期抗逆转录病毒治疗如何阻止抗体的产生,我们分析了急性HIV-1感染不同阶段收集的淋巴结活检中Tfh和B细胞的频率和激活状态。我们的数据表明,早期ART启动后抗体发展下降可能是由于治疗时生发中心发育有限,而靶向CXCR3+ Tfh激活的新干预措施可能有利于增加长期的hiv特异性抗体水平。
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来源期刊
Journal of Virology
Journal of Virology 医学-病毒学
CiteScore
10.10
自引率
7.40%
发文量
906
审稿时长
1 months
期刊介绍: Journal of Virology (JVI) explores the nature of the viruses of animals, archaea, bacteria, fungi, plants, and protozoa. We welcome papers on virion structure and assembly, viral genome replication and regulation of gene expression, genetic diversity and evolution, virus-cell interactions, cellular responses to infection, transformation and oncogenesis, gene delivery, viral pathogenesis and immunity, and vaccines and antiviral agents.
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