Sprouting and network data assessed in the chicken aortic ring assay under stimulation with human adipose-derived stem cell secretomes harvested from single cells or spheroids of different sizes

Abstract

The aortic ring assay is widely used to assess the angiogenic modulation evoked by various drugs. However, different parameters are used as readouts and there is no congruence in terminology. In addition, some researchers use one set of parameters, and other researchers choose parameters that overlap to some extent, but miss a part of these parameters, rendering a direct comparison of the readouts and results difficult [1]. Therefore, we present data acquired in the chicken aortic ring assay that cover the full spectrum of possible readouts – exemplified by stimulation with secretomes harvested from human adipose-derived stem cells, cultivated either as single cell monolayer culture or as 3D spheroids, sized 250 cells/spheroid or 8000 cells/spheroid, respectively.