Relevance of oncobiome in breast cancer evolution in an Argentine cohort.

IF 3.7 2区 生物学 Q2 MICROBIOLOGY
mSphere Pub Date : 2025-03-25 Epub Date: 2025-02-10 DOI:10.1128/msphere.00597-24
Leonardo Néstor Rubén Dandeu, Joel Lachovsky, Sofía Sidlik, Pablo Marenco, Daniela Orschanski, Pablo Aguilera, Martín Vázquez, María Del Pilar Carballo, Elmer Fernández, Alberto Penas-Steinhardt, Norma Alejandra Chasseing, Vivian Labovsky
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引用次数: 0

Abstract

Breast cancer is the leading cause of cancer deaths in women worldwide, with about 20,000 cases annually in Argentina. While age, diet, and genetics are known risk factors, most breast cancer cases have unknown causes, necessitating the discovery of new risk factors. The aim of this study was the analysis of the prognostic relevance of the oncobiome in Argentinean breast cancer patients. Sequencing of the V4 region 16S rRNA gene was performed on 34 primary breast tumor samples, using bioinformatic and statistical analyses to identify bacteria and hypothetical pathways. Each sample presented a unique microbial profile, with Proteobacteria being the most abundant phylum. Tumors >2 cm showed greater alpha diversity with increased nucleotide biosynthesis. Moreover, progesterone-receptor tumors showed differences in beta diversity, being progesterone receptor-positive tumors that had the highest expression of Acinetobacter and Moraxella. In disease progression, the phylum Chloroflexi was prevalent in tumors of surviving patients. Acinetobacter and Cloacibacterium genera were significantly higher in patients without events and those without metastasis. We found that nucleotide and cell-structure biosynthesis, and lipid metabolism pathways were enriched in tumors with poor progression, whereas amino-acid degradation was increased in tumors of surviving patients. This finding is an indication that tumor cells are taking advantage of this effect of the microbiome during tumor progression. We conclude that oncobiome is dysbiotic in these patients, with distinct patterns in those with poor progression. Suggesting a link between the oncobiome and cancer progression, paving the way for new therapies to improve patient quality of life and survival.

Importance: This is the first study to investigate the relevance of the oncobiome in the evolution of breast cancer in a cohort of Argentine patients. It also highlights the need for further research in this area to improve our understanding of the role of the microbiome in this disease and potentially identify new therapeutic targets or prognostic indicators. Understanding the complex interaction between the microbiome, the tumor microenvironment, and the pathogenesis of breast cancer holds the promise of more personalized and effective treatment approaches in the future.

阿根廷队列中肿瘤组与乳腺癌进化的相关性
乳腺癌是全世界妇女癌症死亡的主要原因,阿根廷每年约有20 000例病例。虽然年龄、饮食和遗传是已知的风险因素,但大多数乳腺癌病例的原因未知,因此有必要发现新的风险因素。本研究的目的是分析阿根廷乳腺癌患者肿瘤组的预后相关性。对34例乳腺原发肿瘤样本进行V4区16S rRNA基因测序,利用生物信息学和统计学分析鉴定细菌和假设途径。每个样品都呈现出独特的微生物特征,其中变形菌门是最丰富的门。随着核苷酸生物合成的增加,bbb2cm的肿瘤显示出更大的α多样性。此外,孕激素受体肿瘤在β多样性上存在差异,孕激素受体阳性的肿瘤中不动杆菌和莫拉菌的表达量最高。在疾病进展中,存活患者的肿瘤中普遍存在氯霉素门。在无事件和无转移的患者中,不动杆菌属和cloacacibacterium属的含量明显较高。我们发现,在进展不良的肿瘤中,核苷酸和细胞结构生物合成以及脂质代谢途径丰富,而在存活患者的肿瘤中,氨基酸降解增加。这一发现表明肿瘤细胞在肿瘤进展过程中利用了微生物组的这种作用。我们的结论是,这些患者的肿瘤菌群是不稳定的,在进展不良的患者中有不同的模式。提示肿瘤组与癌症进展之间的联系,为改善患者生活质量和生存率的新疗法铺平了道路。重要性:这是首次在阿根廷患者队列中调查肿瘤组在乳腺癌演变中的相关性。这也强调了在这一领域进一步研究的必要性,以提高我们对微生物组在这种疾病中的作用的理解,并有可能确定新的治疗靶点或预后指标。了解微生物组、肿瘤微环境和乳腺癌发病机制之间复杂的相互作用,有助于在未来找到更加个性化和有效的治疗方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
mSphere
mSphere Immunology and Microbiology-Microbiology
CiteScore
8.50
自引率
2.10%
发文量
192
审稿时长
11 weeks
期刊介绍: mSphere™ is a multi-disciplinary open-access journal that will focus on rapid publication of fundamental contributions to our understanding of microbiology. Its scope will reflect the immense range of fields within the microbial sciences, creating new opportunities for researchers to share findings that are transforming our understanding of human health and disease, ecosystems, neuroscience, agriculture, energy production, climate change, evolution, biogeochemical cycling, and food and drug production. Submissions will be encouraged of all high-quality work that makes fundamental contributions to our understanding of microbiology. mSphere™ will provide streamlined decisions, while carrying on ASM''s tradition for rigorous peer review.
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