GPR180 Reduces Adiposity by Inhibiting Lipogenesis and Fatty Acid Uptake in Adipocytes.

Abstract

Objective: In this study, we examined the effect of Gpr180, a G protein-coupled receptor (GPCR) family member, on lipid metabolism of adipose tissue. Methods: We utilized adeno-associated virus overexpression of Gpr180 in subcutaneous adipose tissue, adipocyte-specific Gpr180 knockout mice and stromal vascular fraction (SVF) cells to explore the role and mechanism of GPR180 in lipid metabolism in adipocytes. Results: Levels of Gpr180 mRNA in subcutaneous and epididymal adipose tissues were significantly reduced in mice fed high fat diet (HFD). Over-expression of Gpr180 in subcutaneous white adipose tissue (sWAT) improved lipid metabolism and protected mice from HFD-induced obesity. Conversely, adipocyte-specific knockout of Gpr180 exacerbated lipid metabolism disorders induced by HFD. In cultured adipocytes differentiated from SVF cells, GPR180 inhibited lipogenesis and fatty acid (FA) uptake. Conclusions: Collectively, our study reveals that GPR180 functions to suppress lipid accumulation in adipocytes.