Whole genome analysis of Planococcus chinensis MB653 isolated from an infected orthopaedic implant sample from Pakistan

Abstract

This study presents the isolation and genomic analysis of the strain Planococcus chinensis MB653, which was obtained from an infected orthopaedic implant sample. Strain MB653 grew optimally at pH 7.0 and at 30–37 °C. The genome of MB653 was sequenced using Illumina sequencing, and the resulting contigs were annotated, with 46.5 % G + C content. The analysis revealed the presence of various virulence factors, including genes involved in protein secretion systems and secretion pathways. Additionally, the strain possessed genes related to flagella and pili, although it was not found to be motile. The whole genome sequence revealed that strain MB653 encoded a larger number of genes associated with antimicrobial resistance than its most other environmental counterpart. The genome analysis identified several antibiotic resistance genes and genes associated with resistance to different compounds. Genes conferring resistance to heavy metals such as cadmium (cadA, cadC), arsenic (arsABC, arsR) and quaternary ammonium compounds (qacC) were also identified. Furthermore, the study explored the secondary metabolite potential of MB653, revealing the presence of gene clusters associated with the production of antimicrobial peptides and terpenes. These findings suggest that MB653 possesses potential virulence factors and may produce secondary metabolites that contribute to its pathogenicity. Further research is needed to investigate the specific roles of these gene clusters and their implications for virulence.