Integrative analysis of genes provides insights into the molecular and immune characteristics of mitochondria-related genes in atherosclerosis

IF 3.4 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics Pub Date : 2025-02-04 DOI:10.1016/j.ygeno.2025.111013

Zhipeng Zheng , Huimin Lu , Xiaowen Wang , Zhiyuan Yang , Yubin Zhang , Kaiyuan Li , Cheng Shen , Zhifeng Yin , Min Sha , Jun Ye , Li Zhu

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引用次数: 0

Abstract

Atherosclerosis is a chronic inflammatory disease characterized by lipid accumulation in arterial walls. The role of the interplay between mitochondrial dysfunction and immune inflammation in atherosclerosis is still unclear. This study aimed to investigate the molecular characteristics and immune landscape of mitochondrial hub genes involved in atherosclerosis. Based on bioinformatics analysis, three hub Mitochondria-related DEGs (MitoDEGs), including OXCT1, UCP2, and CPT1B, were screened out and showed good diagnostic performance in identifying atherosclerosis patients and controls. Immune analysis demonstrated strong correlations between hub MitoDEGs and immune cells, such as macrophages and T cells. Additionally, the predicted transcription factors of these hub MitoDEGs were significantly enriched in Th17, Th1 and Th2 cell differentiation signaling pathways. Both cell and animal experiments confirmed the expression trends of OXCT1 and CPT1B observed in the bioinformatics analysis. These hub MitoDEGs may play an important role in coordinating mitochondrial metabolism in the immune inflammation of atherosclerosis.

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基因的综合分析提供了线粒体相关基因在动脉粥样硬化中的分子和免疫特性的见解

动脉粥样硬化是一种以动脉壁脂质积累为特征的慢性炎症性疾病。线粒体功能障碍和免疫炎症之间的相互作用在动脉粥样硬化中的作用尚不清楚。本研究旨在探讨参与动脉粥样硬化的线粒体枢纽基因的分子特征和免疫景观。基于生物信息学分析，筛选出OXCT1、UCP2、CPT1B等3个中心线粒体相关基因（MitoDEGs），对动脉粥样硬化患者和对照组均有较好的诊断效果。免疫分析表明，中心有丝分裂蛋白与免疫细胞（如巨噬细胞和T细胞）之间存在很强的相关性。此外，这些枢纽MitoDEGs的预测转录因子在Th17， Th1和Th2细胞分化信号通路中显著富集。细胞和动物实验均证实了生物信息学分析中观察到的OXCT1和CPT1B的表达趋势。这些中心mitodeg可能在动脉粥样硬化免疫炎症的线粒体代谢协调中发挥重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genomics 生物-生物工程与应用微生物

CiteScore

9.60

自引率

2.30%

发文量

260

审稿时长

60 days

期刊介绍： Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.