A semi-dominant mutation in the gene encoding histidine kinase influences rice morphology.

IF 1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Genes & genetic systems Pub Date : 2025-04-04 Epub Date: 2025-02-06 DOI:10.1266/ggs.24-00223
Kaito Chiba, Takumi Tezuka, Mayo Watanabe, Nobuhiro Nagasawa, Namiko Satoh-Nagasawa
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引用次数: 0

Abstract

Cytokinin plays a major role in the regulation of plant development. It is perceived by receptors with histidine kinase activity to regulate the expression of various transcription factors. In a previous study, we reported a semi-dominant mutant, named adaxial-abaxial bipolar leaf1 (abl1)-d, which exhibited a characteristic feature in the fourth leaf of rice, and that the ABL1 gene encodes a cytokinin receptor with histidine kinase activity. Our further analysis suggested that the abl1-d mutation is associated with an active form of histidine kinase and altered cytokinin signaling. However, it remained unclear whether the abl1-d mutation indeed triggers aberrant cytokinin signaling in rice plants, and how the abl1-d mutation affects developmental processes throughout the life cycle of rice. In the present study, we found that homozygous abl1-1d calli have the capacity to regenerate shoots in the absence of cytokinin, suggesting that the abl1-1d homozygous mutation is associated with constitutive cytokinin signaling in rice. We next examined morphological characteristics of both homozygous and heterozygous abl1-1d plants from the post-germination vegetative phase through to reproduction. The results showed that homozygous abl1-1d plants had a reduced number of panicles and were completely sterile, and that leaf size and the midrib structure were altered. Furthermore, the adaxial-abaxial bipolar leaf, a phenotype that is characteristic of the abl1-1d mutant, has previously been observed to resemble two normal leaves fused together at their abaxial sides. Leaves with this particular phenotype exhibited enhanced photosynthetic efficiency under certain environmental conditions. Thus, the abl1-1d mutation, which results in a putative active form of receptor histidine kinase, affects various developmental traits throughout the rice life cycle, probably due to altered cytokinin signaling.

组氨酸激酶编码基因的半显性突变影响水稻形态。
细胞分裂素在植物发育调控中起着重要作用。它被具有组氨酸激酶活性的受体感知来调节各种转录因子的表达。在之前的研究中,我们报道了一个半显性突变体,命名为adaxial-abaxial bipolar leaf1 (abl1)-d,它在水稻的第四叶中表现出一个特征,ABL基因编码一个具有组氨酸激酶活性的细胞分裂素受体。我们进一步的分析表明,abl1-d突变与组氨酸激酶的活性形式和细胞分裂素信号的改变有关。然而,目前尚不清楚abl1-d突变是否确实会触发水稻植株的异常细胞分裂素信号,以及abl1-d突变如何影响水稻整个生命周期的发育过程。在本研究中,我们发现纯合子abl1-1d愈伤组织在缺乏细胞分裂素的情况下具有再生芽的能力,这表明abl1-1d纯合子突变与水稻细胞分裂素组成信号传导有关。接下来,我们研究了纯合子和杂合子abl1-1d植物从萌发后营养阶段到繁殖阶段的形态特征。结果表明,纯合子ab11 -1d植株穗数减少,完全不育,叶片大小和中脉结构发生改变。此外,abl1-1d突变体的一种表型特征是,其正面-背面双极叶被观察到类似于在其背面融合在一起的两片正常叶子。具有这种特殊表型的叶片在一定的环境条件下表现出更高的光合效率。因此,abl1-1d突变体表达一种被认为是组氨酸受体激酶的活性形式,可能由于细胞分裂素信号的改变而影响水稻整个生命周期的各种发育性状。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Genes & genetic systems
Genes & genetic systems 生物-生化与分子生物学
CiteScore
1.50
自引率
0.00%
发文量
22
审稿时长
>12 weeks
期刊介绍: Genes & Genetic Systems , formerly the Japanese Journal of Genetics , is published bimonthly by the Genetics Society of Japan.
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