Electrophysiological properties of melanin-concentrating hormone neuron subpopulations defined by anatomical localization and CART expression.

IF 4.2 3区 医学 Q2 NEUROSCIENCES
Frontiers in Cellular Neuroscience Pub Date : 2025-01-22 eCollection Date: 2024-01-01 DOI:10.3389/fncel.2024.1439752
Rafiat Damilola Adekunle, Mohammed Sohel Chowdhury, Lisa Z Fang, Michiru Hirasawa
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引用次数: 0

Abstract

Introduction: Melanin-concentrating hormone (MCH) neurons are essential regulators of energy and glucose homeostasis, sleep-wake behaviors, motivation, learning and memory. These neurons are anatomically distributed across the medial (MH) and lateral hypothalamus (LH), and the adjacent zona incerta (ZI), which may represent functional subgroups with distinct connectivity with different brain regions. Furthermore, MCH neurons can be classified according to co-expression of neuropeptides, such as cocaine and amphetamine- regulated transcript (CART).

Methods: To identify functional similarities and differences of MCH subpopulations, we characterized their intrinsic electrophysiological properties using whole cell current clamp recording on acute brain slices from male and female mice.

Results: MCH neurons were classified into subgroups according to their anatomical localization in three MCH-rich brain areas: MH, LH and ZI. Among the three brain regions, ZI MCH neurons were the least excitable while LH MCH neurons were the most excitable. Furthermore, grouping MCH neurons according to CART co-expression revealed that MCH/CART- cells are uniquely depolarized and excitable, and display H-currents. These MCH/CART- cells were mainly found in the LH, which may in part explain why LH MCH neurons are more excitable. While some sex differences were found, the majority of parameters investigated were not different.

Discussion: Our results suggest that MCH/CART- cells are electrophysiologically distinct, whereas MCH/CART+ cells are largely similar despite their diffuse distribution in the hypothalamus. It is therefore a combination of intrinsic electrophysiological properties and neurochemical identities, in addition to anatomy and connectivity that are likely to be critical in defining functional subpopulations of MCH neurons.

由解剖定位和CART表达定义的黑色素浓缩激素神经元亚群的电生理特性。
黑色素集中激素(melanin - concentration hormone, MCH)神经元是能量和葡萄糖稳态、睡眠-觉醒行为、动机、学习和记忆的重要调节因子。这些神经元在解剖学上分布于下丘脑内侧(MH)和外侧(LH),以及相邻的不动带(ZI),它们可能代表着与不同大脑区域具有明显连通性的功能亚群。此外,MCH神经元可以根据神经肽的共表达进行分类,如可卡因和安非他明调节转录物(CART)。方法:利用全细胞电流箝法记录雄性和雌性小鼠急性脑切片,鉴定MCH亚群的功能异同。结果:根据MCH神经元在MH、LH和ZI三个富含MCH脑区的解剖定位,将MCH神经元分为亚群。在三个脑区中,ZI MCH神经元最不兴奋,LH MCH神经元最兴奋。此外,根据CART共表达对MCH神经元进行分组发现,MCH/CART-细胞具有独特的去极化和兴奋性,并显示h电流。这些MCH/CART-细胞主要存在于LH,这可能部分解释了为什么LH的MCH神经元更容易兴奋。虽然发现了一些性别差异,但调查的大多数参数没有差异。讨论:我们的研究结果表明,MCH/CART-细胞在电生理上是不同的,而MCH/CART+细胞尽管在下丘脑弥漫性分布,但在很大程度上是相似的。因此,除了解剖学和连接性之外,它还结合了内在的电生理特性和神经化学特性,这可能是定义MCH神经元功能亚群的关键。
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来源期刊
CiteScore
7.90
自引率
3.80%
发文量
627
审稿时长
6-12 weeks
期刊介绍: Frontiers in Cellular Neuroscience is a leading journal in its field, publishing rigorously peer-reviewed research that advances our understanding of the cellular mechanisms underlying cell function in the nervous system across all species. Specialty Chief Editors Egidio D‘Angelo at the University of Pavia and Christian Hansel at the University of Chicago are supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.
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