Naoxintong Is Involved in the Coagulation Regulation of Warfarin Through the MAPK Pathway.

IF 1.8 4区医学 Q3 PHARMACOLOGY & PHARMACY

Pharmacogenomics & Personalized Medicine Pub Date : 2025-01-31 eCollection Date: 2025-01-01 DOI:10.2147/PGPM.S489820

Xiao Luo, Ling Chen, Jingsong Xu, Juxiang Li

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引用次数: 0

Abstract

Objective: To explore the effect of Naoxintong (NXT) on warfarin anticoagulation therapy and its potential mechanism.

Methods: TCSMP, SwissTargetprediction, SuperPred, SEA, and Batmanic-TCM were used to search for active ingredients and targets of NXT and warfarin; the DisGENT database was used to find disease targets of coagulation disorders. Cytoscape software was applied to construct the "drug-target"network; the protein interaction network (PPI) was used to study the protein-protein interaction. GO and KEGG were used for functional analysis. The effect of NXT on warfarin anticoagulation was then tested in rats by analyzing coagulation factors in blood before and after drug administration. The expression of MAPK in the liver tissue was determined by Western blot.

Results: The top five components of NXT affecting warfarin anticoagulation degree value were MOL000098, MOL000422, MOL000006, MOL000358, and MOL000449. TP53, AKT1, SRC, TNF, HSP90AA1, STAT3, JUN, IL6, EGFR, and ESR1 were the core targets of NXT, while MAPK9, MAP3K5, MAPK8, and MAPK1 in the MAPK family were important targets of NXT in the coagulation process. In vivo testing indicated that NXT may be able to participate in the regulation of the warfarin coagulation process through multiple targets and multiple pathways, which may be related to MAPK.

Conclusion: Our data suggests that NXT is involved in the coagulation regulation of warfarin through the MAPK pathway.

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脑心通通过MAPK通路参与华法林凝血调节

目的：探讨脑心通对华法林抗凝治疗的影响及其可能机制。方法：采用TCSMP、SwissTargetprediction、SuperPred、SEA和Batmanic-TCM对NXT和华法林的有效成分和靶点进行筛选；使用DisGENT数据库寻找凝血障碍的疾病靶点。应用Cytoscape软件构建“药物靶点”网络；利用蛋白相互作用网络（PPI）研究蛋白-蛋白相互作用。GO和KEGG进行功能分析。通过分析给药前后血凝因子，观察NXT对华法林抗凝作用的影响。Western blot检测MAPK在肝组织中的表达。结果：NXT影响华法林抗凝度值前5位的成分分别为MOL000098、MOL000422、MOL000006、MOL000358、MOL000449。TP53、AKT1、SRC、TNF、HSP90AA1、STAT3、JUN、IL6、EGFR、ESR1是NXT的核心靶点，而MAPK家族中的MAPK9、MAP3K5、MAPK8、MAPK1是NXT在凝血过程中的重要靶点。体内实验表明，NXT可能通过多靶点、多途径参与华法林凝血过程的调节，这可能与MAPK有关。结论：我们的数据表明NXT通过MAPK途径参与华法林的凝血调节。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pharmacogenomics & Personalized Medicine Biochemistry, Genetics and Molecular Biology-Molecular Medicine

CiteScore

3.30

自引率

5.30%

发文量

110

审稿时长

16 weeks

期刊介绍： Pharmacogenomics and Personalized Medicine is an international, peer-reviewed, open-access journal characterizing the influence of genotype on pharmacology leading to the development of personalized treatment programs and individualized drug selection for improved safety, efficacy and sustainability. In particular, emphasis will be given to: Genomic and proteomic profiling Genetics and drug metabolism Targeted drug identification and discovery Optimizing drug selection & dosage based on patient''s genetic profile Drug related morbidity & mortality intervention Advanced disease screening and targeted therapeutic intervention Genetic based vaccine development Patient satisfaction and preference Health economic evaluations Practical and organizational issues in the development and implementation of personalized medicine programs.