Lysophosphatidic acid regulates implant osseointegration in murine models via YAP.

IF 2.8 4区医学 Q3 CELL BIOLOGY

Connective Tissue Research Pub Date : 2025-03-01 Epub Date: 2025-02-04 DOI:10.1080/03008207.2025.2459856

Qin Zhang, Ying Yuan, Bin Wang, Ping Gong, Lin Xiang

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引用次数: 0

Abstract

Background: Lysophosphatidic acid (LPA), a simple bioactive lysophospholipid, has been reported to regulate bone homeostasis and bone remodeling. This study aimed to elucidate the function and intrinsic mechanism of LPA in osseointegration in murine models.

Method: We constructed immediate implant models in murine maxillae. Micro-CT, H&E staining, and PCR assays were performed to evaluate the effects of LPA on osseointegration. Furthermore, Prx1-Cre;Yap^f/f mice and Sp7-Cre;Yap^f/f mice were generated to investigate the role of YAP on LPA-induced osseointegration.

Result: In this study, we identified that LPA might promote bone deposition on the tissue-implant interface and improve osseointegration. In addition, conditional knockout of YAP from MCSs and pre-osteoblasts blunts LPA-induced osteogenesis and osseointegration in mice.

Conclusion: Our data demonstrated that LPA-YAP signaling is particularly important to regulate osseointegration, which expands our understanding of LPA and provide the potential of LPA to be used in osseointegration.

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溶血磷脂酸通过YAP调节小鼠种植体骨整合。

背景：溶血磷脂酸（LPA）是一种简单的生物活性溶血磷脂，据报道可调节骨稳态和骨重塑。本研究旨在阐明LPA在小鼠骨整合中的作用及其内在机制。方法：建立上颌即刻种植体模型。采用Micro-CT、H&E染色和PCR检测评价LPA对骨整合的影响。此外，通过生成Prx1-Cre；Yapf/f小鼠和Sp7-Cre；Yapf/f小鼠来研究YAP在lpa诱导的骨整合中的作用。结果：在本研究中，我们发现LPA可以促进组织-种植体界面的骨沉积，改善骨整合。此外，从MCSs和成骨前细胞中有条件地敲除YAP会使lpa诱导的小鼠成骨和骨整合变得迟钝。结论：我们的数据表明LPA- yap信号在调节骨整合中特别重要，这扩大了我们对LPA的理解，并提供了LPA在骨整合中应用的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Connective Tissue Research 生物-细胞生物学

CiteScore

6.60

自引率

3.40%

发文量

审稿时长

2 months

期刊介绍： The aim of Connective Tissue Research is to present original and significant research in all basic areas of connective tissue and matrix biology. The journal also provides topical reviews and, on occasion, the proceedings of conferences in areas of special interest at which original work is presented. The journal supports an interdisciplinary approach; we present a variety of perspectives from different disciplines, including Biochemistry Cell and Molecular Biology Immunology Structural Biology Biophysics Biomechanics Regenerative Medicine The interests of the Editorial Board are to understand, mechanistically, the structure-function relationships in connective tissue extracellular matrix, and its associated cells, through interpretation of sophisticated experimentation using state-of-the-art technologies that include molecular genetics, imaging, immunology, biomechanics and tissue engineering.