Integrated omics profiles for exploring the potential mechanism underlying aroma formation in the terpenoid-rich aromatic plant Opisthopappus taihangensis and the bioactivity of its leaf essential oil

Maolin Liu , Yushu Li , Haixia Chen , Chunjie He , Lei Sun , Xiuhai Zhang , Zongda Xu , Hua Liu
{"title":"Integrated omics profiles for exploring the potential mechanism underlying aroma formation in the terpenoid-rich aromatic plant Opisthopappus taihangensis and the bioactivity of its leaf essential oil","authors":"Maolin Liu ,&nbsp;Yushu Li ,&nbsp;Haixia Chen ,&nbsp;Chunjie He ,&nbsp;Lei Sun ,&nbsp;Xiuhai Zhang ,&nbsp;Zongda Xu ,&nbsp;Hua Liu","doi":"10.1016/j.agrcom.2024.100061","DOIUrl":null,"url":null,"abstract":"<div><div><em>Opisthopappus taihangensis</em> (Anthemideae, Asteraceae), which is rich in bioactive components, produces flowers and leaves with robust fragrances. In this study, we conducted comprehensive metabolomic and transcriptomic analyses to identify changes in terpenoid metabolites and associated gene expression across various <em>O. taihangensis</em> tissues (leaves, buds, and inflorescences at the exposure, initial-bloom, and full-bloom stages). We identified 1370 metabolites using headspace solid-phase micro-extraction/gas chromatography-mass spectrometry (HS-SPME/GC–MS), 308 of which were terpenoid metabolites. The expression of terpenoid synthesis-related genes was relatively consistent with the synthesis of terpenoid metabolites at each examined developmental stage. An analysis of gene networks governing terpenoid synthesis revealed that <em>MCT</em> genes (<em>OtMCT1</em>, <em>OtMCT2</em>, and <em>OtMCT3</em>), <em>TPS</em> genes (<em>OtTPS5</em>, <em>OtTPS9</em>, and <em>OtTPS10</em>), and <em>OtISPS1</em> may be crucial for synthesizing specific metabolites in different tissues. Additionally, the essential oil extracted from leaves by water distillation showed that thujone and camphor were the predominant components. The considerable antioxidant activity of the leaf essential oil was comparable to that of vitamin C (16 ​μg/mL). Notably, its antimicrobial effects against <em>Staphylococcus aureus</em> and <em>Escherichia coli</em> growth were greater than those of ampicillin and vancomycin at the same concentrations. Scanning electron microscopy images indicated that the leaf essential oil significantly disrupted bacterial cell structures. This study thoroughly analyzed the network of genes regulating terpenoid metabolites in different <em>O. taihangensis</em> tissues, and elucidated the antioxidant and antibacterial potential of the leaf essential oil, thus providing valuable insights for breeding, molecular characterization, and the potential application of <em>O. taihangensis</em> in developing useful essential oil-based natural products.</div></div>","PeriodicalId":100065,"journal":{"name":"Agriculture Communications","volume":"2 4","pages":"Article 100061"},"PeriodicalIF":0.0000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Agriculture Communications","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2949798124000371","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Opisthopappus taihangensis (Anthemideae, Asteraceae), which is rich in bioactive components, produces flowers and leaves with robust fragrances. In this study, we conducted comprehensive metabolomic and transcriptomic analyses to identify changes in terpenoid metabolites and associated gene expression across various O. taihangensis tissues (leaves, buds, and inflorescences at the exposure, initial-bloom, and full-bloom stages). We identified 1370 metabolites using headspace solid-phase micro-extraction/gas chromatography-mass spectrometry (HS-SPME/GC–MS), 308 of which were terpenoid metabolites. The expression of terpenoid synthesis-related genes was relatively consistent with the synthesis of terpenoid metabolites at each examined developmental stage. An analysis of gene networks governing terpenoid synthesis revealed that MCT genes (OtMCT1, OtMCT2, and OtMCT3), TPS genes (OtTPS5, OtTPS9, and OtTPS10), and OtISPS1 may be crucial for synthesizing specific metabolites in different tissues. Additionally, the essential oil extracted from leaves by water distillation showed that thujone and camphor were the predominant components. The considerable antioxidant activity of the leaf essential oil was comparable to that of vitamin C (16 ​μg/mL). Notably, its antimicrobial effects against Staphylococcus aureus and Escherichia coli growth were greater than those of ampicillin and vancomycin at the same concentrations. Scanning electron microscopy images indicated that the leaf essential oil significantly disrupted bacterial cell structures. This study thoroughly analyzed the network of genes regulating terpenoid metabolites in different O. taihangensis tissues, and elucidated the antioxidant and antibacterial potential of the leaf essential oil, thus providing valuable insights for breeding, molecular characterization, and the potential application of O. taihangensis in developing useful essential oil-based natural products.
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信