Genotoxic and cytotoxic effects of polystyrene nanoplastics on human lymphocytes: A comprehensive analysis

IF 2.3 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Mutation research. Genetic toxicology and environmental mutagenesis Pub Date : 2025-02-01 DOI:10.1016/j.mrgentox.2025.503850

Ahmet Ali Berber , Nihan Akinci Kenanoğlu , Şefika Nur Demi̇r , Hüseyin Aksoy

{"title":"Genotoxic and cytotoxic effects of polystyrene nanoplastics on human lymphocytes: A comprehensive analysis","authors":"Ahmet Ali Berber , Nihan Akinci Kenanoğlu , Şefika Nur Demi̇r , Hüseyin Aksoy","doi":"10.1016/j.mrgentox.2025.503850","DOIUrl":null,"url":null,"abstract":"<div><div>A growing amount of plastic waste is finding its way into natural ecosystems as a result of the widespread usage of plastics in modern society. These wastes degrade physically and biologically over time, transforming into microplastics (MPs) and nanoplastics (NPs). MPs and NPs emissions from the terrestrial environment then mix with rivers and eventually the seas, forming garbage. The cytotoxic and genotoxic effects of 50 nm polystyrene nanoplastics (PsNP) on human lymphocytes were assessed using the in vitro mitotic index (MI), micronucleus (MN), and comet assays. Both 24 and 48-h applications were performed for MI, and it was determined that 50 nm PsNP provided a statistically significant decrease in MI compared to the control at all concentrations and application times (except 0.001 and 0.1 μg/mL at 24 h). According to the MN test results, the MN frequency increased significantly at all concentrations when compared to the negative control. In the comet test, a statistically significant increase of comet tail length was observed at 0.001, 10 and 100 μg/mL concentration with 50 nm PsNP exposure. Tail moment also showed a statistically significant increase at the lowest concentration of 0.001 μg/mL and the highest concentration of 1, 10, 100 μg/mL compared to the negative control. All test results show that PsNP has both genotoxic and cytotoxic potential.</div></div>","PeriodicalId":18799,"journal":{"name":"Mutation research. Genetic toxicology and environmental mutagenesis","volume":"902 ","pages":"Article 503850"},"PeriodicalIF":2.3000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mutation research. Genetic toxicology and environmental mutagenesis","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1383571825000099","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

A growing amount of plastic waste is finding its way into natural ecosystems as a result of the widespread usage of plastics in modern society. These wastes degrade physically and biologically over time, transforming into microplastics (MPs) and nanoplastics (NPs). MPs and NPs emissions from the terrestrial environment then mix with rivers and eventually the seas, forming garbage. The cytotoxic and genotoxic effects of 50 nm polystyrene nanoplastics (PsNP) on human lymphocytes were assessed using the in vitro mitotic index (MI), micronucleus (MN), and comet assays. Both 24 and 48-h applications were performed for MI, and it was determined that 50 nm PsNP provided a statistically significant decrease in MI compared to the control at all concentrations and application times (except 0.001 and 0.1 μg/mL at 24 h). According to the MN test results, the MN frequency increased significantly at all concentrations when compared to the negative control. In the comet test, a statistically significant increase of comet tail length was observed at 0.001, 10 and 100 μg/mL concentration with 50 nm PsNP exposure. Tail moment also showed a statistically significant increase at the lowest concentration of 0.001 μg/mL and the highest concentration of 1, 10, 100 μg/mL compared to the negative control. All test results show that PsNP has both genotoxic and cytotoxic potential.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Mutation research. Genetic toxicology and environmental mutagenesis 生物-毒理学

CiteScore

3.80

自引率

5.30%

发文量

审稿时长

105 days

期刊介绍： Mutation Research - Genetic Toxicology and Environmental Mutagenesis (MRGTEM) publishes papers advancing knowledge in the field of genetic toxicology. Papers are welcomed in the following areas: New developments in genotoxicity testing of chemical agents (e.g. improvements in methodology of assay systems and interpretation of results). Alternatives to and refinement of the use of animals in genotoxicity testing. Nano-genotoxicology, the study of genotoxicity hazards and risks related to novel man-made nanomaterials. Studies of epigenetic changes in relation to genotoxic effects. The use of structure-activity relationships in predicting genotoxic effects. The isolation and chemical characterization of novel environmental mutagens. The measurement of genotoxic effects in human populations, when accompanied by quantitative measurements of environmental or occupational exposures. The application of novel technologies for assessing the hazard and risks associated with genotoxic substances (e.g. OMICS or other high-throughput approaches to genotoxicity testing). MRGTEM is now accepting submissions for a new section of the journal: Current Topics in Genotoxicity Testing, that will be dedicated to the discussion of current issues relating to design, interpretation and strategic use of genotoxicity tests. This section is envisaged to include discussions relating to the development of new international testing guidelines, but also to wider topics in the field. The evaluation of contrasting or opposing viewpoints is welcomed as long as the presentation is in accordance with the journal''s aims, scope, and policies.