DART-HRMS analysis as a screening method for hallucinogenic mushrooms

IF 2.6 3区医学 Q2 CHEMISTRY, ANALYTICAL

Forensic Chemistry Pub Date : 2024-12-05 DOI:10.1016/j.forc.2024.100629

Vanessa Braga Galhego, Raquel Capilla Loureiro Alves de Santana, Cecília de Andrade Bhering, Ananda da Silva Antonio, Gabriela Vanini

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引用次数: 0

Abstract

The global drug abuse scene is dynamic. After COVID-19 psilocin-containing mushrooms (PCM) reemerged in the international scenario. To identify illicit drugs, multiple analytical methods must be employed including triage (e.g. colorimetric test) and confirmatory tests (e.g. GC–MS). In addition, in many cases, only samples that give positive results in the triage method are taken to the confirmatory ones. However, colorimetric methods have several drawbacks in the analysis of complex unusual samples, such as the PCM, which can produce false-negative results. This study evaluated the feasibility of the ambient ionization technique direct analysis in real time – high-resolution mass spectrometry (DART-HRMS) as a triage method for the identification of PCM seized samples. A psilocin standard, edible mushrooms, and 5 seized PCM samples were used to establish the DART-HRMS instrumental setup required for psilocin analysis. The developed DART-HRMS method was compared to the triage method recommended for PCM screening analysis (Erlich’s test). While the analysis of psilocin solutions applied 300 V at the DART grid and carrier gas at 150 °C, the analysis of solid samples applied 300 V and 375 °C. All seized PCM samples presented negative results in Erlich’s test, while all of them were positive for psilocin by DART-HRMS. Based on the quality of the HRMS data it was possible to confirm that Erlich’s test results were false-negative for the seized samples. DART-HRMS presents itself as a fast, reliable, and trustworthy triage method for the qualitative identification of raw hallucinogenic mushrooms for criminal investigation.

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来源期刊

Forensic Chemistry CHEMISTRY, ANALYTICAL-

CiteScore

5.70

自引率

14.80%

发文量

审稿时长

46 days

期刊介绍： Forensic Chemistry publishes high quality manuscripts focusing on the theory, research and application of any chemical science to forensic analysis. The scope of the journal includes fundamental advancements that result in a better understanding of the evidentiary significance derived from the physical and chemical analysis of materials. The scope of Forensic Chemistry will also include the application and or development of any molecular and atomic spectrochemical technique, electrochemical techniques, sensors, surface characterization techniques, mass spectrometry, nuclear magnetic resonance, chemometrics and statistics, and separation sciences (e.g. chromatography) that provide insight into the forensic analysis of materials. Evidential topics of interest to the journal include, but are not limited to, fingerprint analysis, drug analysis, ignitable liquid residue analysis, explosives detection and analysis, the characterization and comparison of trace evidence (glass, fibers, paints and polymers, tapes, soils and other materials), ink and paper analysis, gunshot residue analysis, synthetic pathways for drugs, toxicology and the analysis and chemistry associated with the components of fingermarks. The journal is particularly interested in receiving manuscripts that report advances in the forensic interpretation of chemical evidence. Technology Readiness Level: When submitting an article to Forensic Chemistry, all authors will be asked to self-assign a Technology Readiness Level (TRL) to their article. The purpose of the TRL system is to help readers understand the level of maturity of an idea or method, to help track the evolution of readiness of a given technique or method, and to help filter published articles by the expected ease of implementation in an operation setting within a crime lab.