DNA interaction and cytotoxicity of a new asymmetric dimer cobalt(II) complex [Co(phen)2(Cl2)]3(H2O).[Co(phen)2(Cl)(H2O)](Cl)

Chemistry of Inorganic Materials Pub Date : 2024-12-01 Epub Date: 2024-10-10 DOI:10.1016/j.cinorg.2024.100071

P. Gurumoorthy , S. Ulaganathan , S. Baskaran

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Abstract

The new, unusual and asymmetric cobalt(II) complex, [Co(phen)₂(Cl₂)]3(H₂O).[Co(phen)₂(Cl)(H₂O)](Cl) (phen = 1,10-phenanthroline), has been synthesized and characterized structurally by regular spectral and single crystal XRD analysis. An asymmetric complex crystallized in a monoclinic system with space group P2₁/c, a = 14.093(5) Å, b = 24.089(5) Å, c = 14.241(5) Å, α = 90.000(5)^°, β = 98.010(5)^°, and γ = 90.000(5)^°. DNA binding interactions of complex with calf thymus DNA (CT-DNA) has investigated by UV–Vis absorption, ethidium bromide displacement assay, viscometric and cyclic voltammetric titrations, and the results infer an intercalation mode of binding. Complex cleaves effectively plasmid pBR322 DNA, and exhibits remarkable cytotoxic activity against human colon cancer cell line (HCT15).

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新型非对称二聚体钴（II）配合物[Co(phen)2(Cl2)]3(H2O).[Co(phen)2(Cl)(H2O)]（Cl）的DNA相互作用和细胞毒性

合成了一种新的、不对称的钴（II）配合物[Co(phen)2(Cl2)]3(H2O).[Co(phen)2(Cl)(H2O)](Cl) （phen = 1,10-菲罗啉），并用规则光谱和单晶XRD分析对其结构进行了表征。非对称复杂结晶在单斜晶系,空间群P21 / c, a = 14.093 (5), b = 24.089 (5) a, c = 14.241(5),α= 90.000(5)°,β= 98.010(5)°,γ= 90.000(5)°。采用紫外可见吸收、溴化乙啶置换法、粘度法和循环伏安滴定法研究了复合物与小牛胸腺DNA （CT-DNA）的结合相互作用，结果表明复合物与小牛胸腺DNA的结合存在嵌入模式。复合物可有效切割pBR322质粒DNA，对人结肠癌细胞系（HCT15）表现出显著的细胞毒活性。

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Chemistry of Inorganic Materials

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