Divergent leaf traits of medicinally important Cocculus hirsutus (L.) Diels- morphoanatomical and molecular authentication

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics Pub Date : 2024-11-30 DOI:10.1016/j.egg.2024.100310

Rakshitha H M. Jain, Y.L. Krishnamurty, K. Manjunath, Shashwathi H S

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Abstract

Cocculus hirsutus (L.) Diels (Menispermaceae) is a perennial liana found mainly in tropical and subtropical regions of the Western Ghats, India. This study investigates the variation in leaf morphology within this species. Five samples were selected based on differences in leaf structure, and morphological analysis of foliar traits (Petiole length, Leaf length and Leaf width) revealed significant variation (p < 0.0001) through one-way ANOVA (F-values for PL = 1247.46, LL = 1303.51, and LW = 1413.46). Principal component analysis (PCA) was used to further explore this diversity. Morphoanatomical features, including leaf epidermis, were examined for accurate species identification. DNA barcoding using matK, rbcL, and combined sequences showed superior results with rbcL and the combined sequence. Maximum likelihood (ML) and Bayesian Inference (BI) were applied to assess species relationships, and the analysis suggested all samples belong to the same species. Nevertheless, discrepancies in the bootstrap and posterior probability values revealed by the rbcL and combined sequences may be attributed to microbial influence or mimicry from the host plant.

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来源期刊

Ecological Genetics and Genomics Agricultural and Biological Sciences-Ecology, Evolution, Behavior and Systematics

CiteScore

1.80

自引率

0.00%

发文量

期刊介绍： Ecological Genetics and Genomics publishes ecological studies of broad interest that provide significant insight into ecological interactions or/ and species diversification. New data in these areas are published as research papers, or methods and resource reports that provide novel information on technologies or tools that will be of interest to a broad readership. Complete data sets are shared where appropriate. The journal also provides Reviews, and Perspectives articles, which present commentary on the latest advances published both here and elsewhere, placing such progress in its broader biological context. Topics include: -metagenomics -population genetics/genomics -evolutionary ecology -conservation and molecular adaptation -speciation genetics -environmental and marine genomics -ecological simulation -genomic divergence of organisms