Rare Taxa Are Key Links in Regional Antimicrobial Resistance Profiles in Dusts Across Diverse North American Regions

Q1 Agricultural and Biological Sciences
Paul B. L. George, Florent Rossi, Marc Veillette, Amélia Bélanger Cayouette, Samantha Leclerc, Cindy Dumais, Nathalie Turgeon, Caroline Duchaine
{"title":"Rare Taxa Are Key Links in Regional Antimicrobial Resistance Profiles in Dusts Across Diverse North American Regions","authors":"Paul B. L. George,&nbsp;Florent Rossi,&nbsp;Marc Veillette,&nbsp;Amélia Bélanger Cayouette,&nbsp;Samantha Leclerc,&nbsp;Cindy Dumais,&nbsp;Nathalie Turgeon,&nbsp;Caroline Duchaine","doi":"10.1002/edn3.70049","DOIUrl":null,"url":null,"abstract":"<p>The role of bioaerosols in the dispersal of antimicrobial resistance genes (ARGs) and resistant microorganisms is poorly understood. In addition, bioaerosols are powerful composite samples representative of the surrounding environment and can be used as sentinels of many local habitats. Evidence suggests that using environmental DNA from dust collected on vehicle cabin air filters can define regional resistance profiles. Here, this method was used to investigate differences in resistance gene profiles, their underlying bacterial communities, and their links to anthropogenic and environmental variables across Canada. In total, 477 car filter samples were collected, with every province and territory being represented. DNA was extracted from filter dust. High-throughput qPCR was used to detect and quantify a panel of 36 ARGs and 3 mobile genetic elements. Bacterial biomass was assessed using standard qPCR methods of the 16S rRNA gene, which was also used to assess bacterial biodiversity via metabarcoding. Results indicated that <i>qepA</i> dominates antimicrobial resistance profiles across Canada. However, after they were removed from the dataset, regional profiles were evident based on gene type and richness. Factors positively linked to total numbers of ARGs included human and livestock populations; whereas mean annual precipitation was negatively linked to resistance gene quantities. Measures of α-diversity were generally greater in the western regions of Canada than in the east and the north. Community composition analyses showed similarities between the prairies and territories, which were separated from other regions. Finally, network analyses revealed a relatively stable group of core ARGs across regions, which were largely correlated with low-abundance genera. Such findings suggest that rare taxa are key links in the diffusion of antimicrobial resistance in environmental contexts. Furthermore, this study highlights the potential application of vehicle air filters in building long-term monitoring capacity of outdoor bioaerosols.</p>","PeriodicalId":52828,"journal":{"name":"Environmental DNA","volume":"6 6","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/edn3.70049","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental DNA","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/edn3.70049","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0

Abstract

The role of bioaerosols in the dispersal of antimicrobial resistance genes (ARGs) and resistant microorganisms is poorly understood. In addition, bioaerosols are powerful composite samples representative of the surrounding environment and can be used as sentinels of many local habitats. Evidence suggests that using environmental DNA from dust collected on vehicle cabin air filters can define regional resistance profiles. Here, this method was used to investigate differences in resistance gene profiles, their underlying bacterial communities, and their links to anthropogenic and environmental variables across Canada. In total, 477 car filter samples were collected, with every province and territory being represented. DNA was extracted from filter dust. High-throughput qPCR was used to detect and quantify a panel of 36 ARGs and 3 mobile genetic elements. Bacterial biomass was assessed using standard qPCR methods of the 16S rRNA gene, which was also used to assess bacterial biodiversity via metabarcoding. Results indicated that qepA dominates antimicrobial resistance profiles across Canada. However, after they were removed from the dataset, regional profiles were evident based on gene type and richness. Factors positively linked to total numbers of ARGs included human and livestock populations; whereas mean annual precipitation was negatively linked to resistance gene quantities. Measures of α-diversity were generally greater in the western regions of Canada than in the east and the north. Community composition analyses showed similarities between the prairies and territories, which were separated from other regions. Finally, network analyses revealed a relatively stable group of core ARGs across regions, which were largely correlated with low-abundance genera. Such findings suggest that rare taxa are key links in the diffusion of antimicrobial resistance in environmental contexts. Furthermore, this study highlights the potential application of vehicle air filters in building long-term monitoring capacity of outdoor bioaerosols.

Abstract Image

稀有分类群是北美不同地区粉尘区域抗菌素耐药性概况的关键环节
生物气溶胶在抗菌素耐药基因(ARGs)和耐药微生物传播中的作用尚不清楚。此外,生物气溶胶是代表周围环境的强大复合样品,可以用作许多当地栖息地的哨兵。有证据表明,利用车辆舱室空气过滤器收集的灰尘中的环境DNA可以确定区域阻力分布。在这里,这种方法被用来研究抗性基因谱的差异,它们潜在的细菌群落,以及它们与加拿大各地的人为和环境变量的联系。总共收集了477个汽车滤清器样本,每个省和地区都有代表。DNA是从滤尘中提取的。采用高通量qPCR技术对36个ARGs和3个移动遗传元件进行检测和定量。采用16S rRNA基因的标准qPCR方法评估细菌生物量,并通过元条形码评估细菌多样性。结果表明,qepA在加拿大各地的抗菌素耐药谱中占主导地位。然而,将它们从数据集中删除后,基于基因类型和丰富度的区域分布特征很明显。与ARGs总数正相关的因素包括人类和牲畜种群;而年平均降水量与抗性基因数量呈负相关。α-多样性指数在加拿大西部地区普遍高于东部和北部地区。群落组成分析表明,草原和领土之间存在相似性,这些地区与其他地区分开。最后,网络分析显示,不同地区的核心arg群相对稳定,主要与低丰度属相关。这些发现表明,稀有类群是环境背景下抗菌素耐药性扩散的关键环节。此外,本研究强调了车载空气过滤器在建立室外生物气溶胶长期监测能力方面的潜在应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Environmental DNA
Environmental DNA Agricultural and Biological Sciences-Ecology, Evolution, Behavior and Systematics
CiteScore
11.00
自引率
0.00%
发文量
99
审稿时长
16 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信