A Novel Rhodamine-Tetraphenylporphyrin Fluorescence Probe for Imaging Mercury In Vitro and In Vivo

IF 2 3区化学 Q2 CHEMISTRY, ORGANIC

Journal of Heterocyclic Chemistry Pub Date : 2024-10-21 DOI:10.1002/jhet.4922

Ling Li, Yinuo Yao, Xiaowan Wang, Yaoqi Yu, Tailong Ji, Han Li, Yi Li, Yuanyuan Liu

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引用次数: 0

Abstract

A highly selective fluorescent sensor Por-RBO for determination of Hg²⁺ was designed and synthesized with Rhodamine B and Tetraphenylporphyrin. The sensor can determine the Hg²⁺ of a solution within the pH 5.0–8.0, free from interference of other metal ions. When detected in buffer solution, the fluorescence was the strongest when five times the concentration of Hg²⁺ ion was added. Calculated by fluorescence titration method, the detection limit of sensor Por-RBO for Hg²⁺ ion was as low as 0.12 μmol/L. The geometries of Por-RBO and Por-RBO-Hg²⁺ were optimized at the B3LYP/6-31G** level by density functional theory. The charge distribution, orbital interactions, and bonding characteristics were analyzed and compared in detail to discuss the recognition mechanism and structure–fluorescence property relationships. The results of cell fluorescence imaging and CCK-8 experiments indicate that the sensor Por-RBO exhibits lower cytotoxicity. Por-RBO can be used for fluorescence distribution imaging of Hg²⁺ in living cells.

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来源期刊

Journal of Heterocyclic Chemistry 化学-有机化学

CiteScore

5.20

自引率

4.20%

发文量

177

审稿时长

3.9 months

期刊介绍： The Journal of Heterocyclic Chemistry is interested in publishing research on all aspects of heterocyclic chemistry, especially development and application of efficient synthetic methodologies and strategies for the synthesis of various heterocyclic compounds. In addition, Journal of Heterocyclic Chemistry promotes research in other areas that contribute to heterocyclic synthesis/application, such as synthesis design, reaction techniques, flow chemistry and continuous processing, multiphase catalysis, green chemistry, catalyst immobilization and recycling.