Foraminiferal denitrification and deep bioirrigation influence benthic biogeochemical cycling in a seasonally hypoxic fjord

Abstract

Benthic macro- and micro-biota often play significant roles in controlling the biogeochemical dynamics in sediments. Their activity can be influenced by oxygen availability and impacted by the rise in global hypoxia in coastal regions over the last decades. To understand how these organisms interact with coastal hypoxia and influence sediment biogeochemistry, we undertook a study of early diagenesis in Bedford Basin, a seasonally hypoxic fjord on the West Atlantic coast in Nova Scotia, Canada, using a combination of observations and reaction-transport modeling. We observed that the seafloor was a source of ammonium and sink of nitrate with average fluxes of 2.2 ± 1.8 and −0.9 ± 0.7 mmol m⁻² d⁻¹ respectively. The diffusive oxygen uptake was 14 ± 4.6 mmol m⁻² d⁻¹ and the total organic carbon content in collected sediment cores was 5–7 % with a C/N ratio of ∼10. The pyrite content increased steadily from 0.5 wt% Fe at surface to ∼2 wt% Fe at 20 cm depth. Hydrogen sulfide was negligible down to 25 cm depth most of the time. The sediment was inhabited by tube-forming polychaete Spiochaetopterus sp. that formed tubes up to ∼30 cm in length. The living foraminiferal assemblage in the top 5 cm sediment was found to be dominated (>85 %) by nitrate-storing and denitrifying benthic foraminifera Stainforthia fusiformis. These observations were used to develop and constrain a biogeochemical reaction-transport model. The model results suggest that the observed decrease in porewater concentrations of ammonium and dissolved inorganic carbon below 5 cm depth, was due to deep bioirrigation by tubeworms, accounting for almost 50 % of the benthic efflux. The model further revealed that the deep bioirrigation along with bioturbation and iron cycling prevented accumulation of free sulfide in the top 25 cm sediment despite oxygen penetration depths of ∼1 mm. Modelled organic carbon and nitrogen deposition was 25.2 and 2.9 mmol m⁻² d⁻¹ with burial efficiencies of 23 % and 17 %, respectively. The model indicated a total denitrification rate of 1.3 mmol N m⁻² d⁻¹ that was largely (∼70 %) driven by benthic foraminifera. This study reports the first evidence of foraminiferal denitrification in western Atlantic coastal sediments, and suggests that eukaryote mediated denitrification is an important driver of sediment N-loss in seasonally hypoxic environments, a process that has been traditionally assumed to be carried out by prokaryotic microbes.