Live-Cell Single-Molecule Imaging of Influenza A Virus-Receptor Interaction.

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-01-01 DOI:10.1007/978-1-0716-4326-6_4

Lukas Broich, Yang Fu, Christian Sieben

引用次数: 0

Abstract

Influenza A viruses are a major health care burden, and their biology has been intensely studied for decades. However, many details of virus infection are still elusive, requiring the development of refined and advanced technologies. Super-resolution microscopy allows the study of virus replication at the scale of an infecting virus, offering an exciting perspective on previously unseen mechanistic details of infection. Here we describe the materials and procedures required to perform single-molecule imaging of virus-receptor interaction in live cells. We further provide hints and tips on how to analyze and visualize the obtained datasets.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.