Live-Cell Single-Molecule Imaging of Influenza A Virus-Receptor Interaction.

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-01-01 DOI:10.1007/978-1-0716-4326-6_4

Lukas Broich, Yang Fu, Christian Sieben

引用次数: 0

Abstract

Influenza A viruses are a major health care burden, and their biology has been intensely studied for decades. However, many details of virus infection are still elusive, requiring the development of refined and advanced technologies. Super-resolution microscopy allows the study of virus replication at the scale of an infecting virus, offering an exciting perspective on previously unseen mechanistic details of infection. Here we describe the materials and procedures required to perform single-molecule imaging of virus-receptor interaction in live cells. We further provide hints and tips on how to analyze and visualize the obtained datasets.

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甲型流感病毒与受体相互作用的活细胞单分子成像。

甲型流感病毒是一个主要的卫生保健负担，几十年来，人们一直在对其生物学进行深入研究。然而，病毒感染的许多细节仍然难以捉摸，需要发展精细和先进的技术。超分辨率显微镜允许在感染病毒的规模上研究病毒复制，为以前看不见的感染机制细节提供了令人兴奋的视角。在这里，我们描述了在活细胞中进行病毒-受体相互作用的单分子成像所需的材料和程序。我们进一步提供了如何分析和可视化获得的数据集的提示和技巧。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.