Proteo-Transcriptomic Analysis Reveals the Mechanisms Underlying Escherichia coli Phenotypic Shifts Under Blue Light

IF 3.5 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology and Bioengineering Pub Date : 2025-01-28 DOI:10.1002/bit.28939

Shuqi Shi, Wenlu Qi, Jinming Zhang, Caice Liang, Wei Liu, Hui Han, Wei Zhuang, Tianpeng Chen, Wenjun Sun, Yong Chen

引用次数: 0

Abstract

Bacteria can adapt their lifestyles, including microbial growth, metabolism, and biofilm formation, in response to light signaling. However, the molecular pathways through which blue light affects the lifestyle of Escherichia coli (E. coli) remain incomplete and poorly understood. To address this gap, transcriptomic and proteomic approaches were employed to analyze the physiological differences of E. coli under dark and blue light conditions. Our results indicate that, compared to dark conditions, blue light attenuates flagellar assembly, reduces cell motility and communication, and decreases biofilm formation in E. coli. In addition, this study elucidates the signaling pathways involved in the blue light-mediated regulation of E. coli behavior, providing a theoretical framework for understanding how E. coli responds to blue light signaling to modulate biofilm formation for the production of food chemicals.

Abstract Image

查看原文本刊更多论文

细菌可根据光信号调整其生活方式，包括微生物生长、新陈代谢和生物膜形成。然而，蓝光影响大肠杆菌（E. coli）生活方式的分子途径仍不完整，人们对其了解甚少。为了填补这一空白，我们采用了转录组和蛋白质组方法来分析大肠杆菌在黑暗和蓝光条件下的生理差异。我们的研究结果表明，与黑暗条件相比，蓝光削弱了大肠杆菌鞭毛的组装，降低了细胞的运动和交流能力，并减少了生物膜的形成。此外，这项研究还阐明了参与蓝光介导的大肠杆菌行为调节的信号通路，为了解大肠杆菌如何响应蓝光信号调节生物膜形成以生产食品化学品提供了一个理论框架。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biotechnology and Bioengineering 工程技术-生物工程与应用微生物

CiteScore

7.90

自引率

5.30%

发文量

280

审稿时长

2.1 months

期刊介绍： Biotechnology & Bioengineering publishes Perspectives, Articles, Reviews, Mini-Reviews, and Communications to the Editor that embrace all aspects of biotechnology. These include: -Enzyme systems and their applications, including enzyme reactors, purification, and applied aspects of protein engineering -Animal-cell biotechnology, including media development -Applied aspects of cellular physiology, metabolism, and energetics -Biocatalysis and applied enzymology, including enzyme reactors, protein engineering, and nanobiotechnology -Biothermodynamics -Biofuels, including biomass and renewable resource engineering -Biomaterials, including delivery systems and materials for tissue engineering -Bioprocess engineering, including kinetics and modeling of biological systems, transport phenomena in bioreactors, bioreactor design, monitoring, and control -Biosensors and instrumentation -Computational and systems biology, including bioinformatics and genomic/proteomic studies -Environmental biotechnology, including biofilms, algal systems, and bioremediation -Metabolic and cellular engineering -Plant-cell biotechnology -Spectroscopic and other analytical techniques for biotechnological applications -Synthetic biology -Tissue engineering, stem-cell bioengineering, regenerative medicine, gene therapy and delivery systems The editors will consider papers for publication based on novelty, their immediate or future impact on biotechnological processes, and their contribution to the advancement of biochemical engineering science. Submission of papers dealing with routine aspects of bioprocessing, description of established equipment, and routine applications of established methodologies (e.g., control strategies, modeling, experimental methods) is discouraged. Theoretical papers will be judged based on the novelty of the approach and their potential impact, or on their novel capability to predict and elucidate experimental observations.