Cytotoxic Effects Of Lecaniodiscus Cupanioides (Planch.) Extract and Triterpenoids-derived Gold Nanoparticles On MCF-7 Breast Cancer Cell Lines.

IF 2.6 4区 医学 Q3 CHEMISTRY, MEDICINAL
R Magadani, Derek Tantoh Ndinteh, S Roux, Louisiane Patrick Nangah, Item Justin Atangwho, Daniel Ejim Uti, Esther U Alum, Simeon Ikechukwu Egba
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Abstract

Background: The prevalent disease known as breast cancer has a significant impact on both men's and women's health and quality of life.

Aim: The aim of this study was to explore the potential roles of Lecaniodiscus cupanioides (planch.) extract and triterpenoid-derived gold nanoparticles (AuNPs) in cancer therapy, specifically targeting MCF-7 breast cancer cell lines.

Methods: Gold nanoparticles were synthesized utilizing triterpenoid (ZJ-AuNPs) and leaf extract from Lecaniodiscus cupanioides (LC-AuNPs). Fourier transform infrared spectroscopy (FTIR), Dynamic light scattering (DLS), High-resolution transmission electron microscopy (HRTEM), and UV-vis spectroscopy were employed to characterize the nanoparticles. Additionally, the MTT assay was used to assess the impact of AuNPs on cancer cell viability using MCF-7 breast cancer cell lines.

Results: Analysis of ZJ-AuNPs and LC-AuNPs revealed DLS zeta potentials of -31.8 and -35.8 mV, respectively, and a corresponding UV-vis absorption maxima at 540 and 550 nm. Also, the ZJ-AuNPs and LC-AuNPs had respective zeta-sizes that ranged from 25.84 to 35.98 nm and polydispersive index values between 0.2360 and 0.773.Furthermore, the presence of the chemical groups -OH and -NH was shown to be necessary for the green method of capping and reducing the gold nanoparticles. Nevertheless, a significant decrease in cell viability percentages was noted in the MTT experiment, accompanied by an increase in the quantity or concentration of the nanoparticles for both ZJ-AuNPs and LC-AuNPs.

Conclusion: Given the data obtained in this study, the biosynthesized ZJ-AuNPs and LC-AuNPs were shown to possess potent cytotoxic effects on breast cancer cells. Hence, they may be valuable tools in the development of new cancer chemotherapy drugs.

赤芍的细胞毒作用MCF-7乳腺癌细胞系的提取液和三萜衍生金纳米颗粒。
背景:被称为乳腺癌的流行疾病对男性和女性的健康和生活质量都有重大影响。目的:探讨Lecaniodiscus cupanioides (planch.)提取物和三萜衍生金纳米颗粒(AuNPs)在癌症治疗中的潜在作用,特别是针对MCF-7乳腺癌细胞系。方法:以三萜(ZJ-AuNPs)和Lecaniodiscus cupanioides叶提取物(LC-AuNPs)为原料合成纳米金。采用傅里叶变换红外光谱(FTIR)、动态光散射(DLS)、高分辨率透射电子显微镜(HRTEM)和紫外可见光谱(UV-vis)对纳米颗粒进行表征。此外,MTT试验用于评估AuNPs对MCF-7乳腺癌细胞系癌细胞活力的影响。结果:ZJ-AuNPs和LC-AuNPs的DLS zeta电位分别为-31.8和-35.8 mV,在540和550 nm处最大紫外可见吸收。ZJ-AuNPs和LC-AuNPs的ζ大小在25.84 ~ 35.98 nm之间,多色散指数在0.2360 ~ 0.773之间。此外,化学基团-OH和-NH的存在被证明是绿盖和还原金纳米粒子的必要条件。然而,在MTT实验中发现细胞存活率明显下降,同时ZJ-AuNPs和LC-AuNPs的纳米颗粒数量或浓度增加。结论:根据本研究获得的数据,生物合成的ZJ-AuNPs和LC-AuNPs对乳腺癌细胞具有很强的细胞毒作用。因此,它们可能是开发新的癌症化疗药物的有价值的工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Anti-cancer agents in medicinal chemistry
Anti-cancer agents in medicinal chemistry ONCOLOGY-CHEMISTRY, MEDICINAL
CiteScore
5.10
自引率
3.60%
发文量
323
审稿时长
4-8 weeks
期刊介绍: Formerly: Current Medicinal Chemistry - Anti-Cancer Agents. Anti-Cancer Agents in Medicinal Chemistry aims to cover all the latest and outstanding developments in medicinal chemistry and rational drug design for the discovery of anti-cancer agents. Each issue contains a series of timely in-depth reviews and guest edited issues written by leaders in the field covering a range of current topics in cancer medicinal chemistry. The journal only considers high quality research papers for publication. Anti-Cancer Agents in Medicinal Chemistry is an essential journal for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important developments in cancer drug discovery.
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