Sex steroid disrupting effects of nonsteroidal anti-inflammatory drugs on the Persian Gulf Arabian Sea bream, Acanthopagrus arabicus: In vitro model of environmental drug contamination

IF 2.6 3区医学 Q3 TOXICOLOGY

Toxicology in Vitro Pub Date : 2025-01-24 DOI:10.1016/j.tiv.2025.106008

Zahra Beitgader , Negin Salamat , Mohammad Ali Salarialiabadi , Hoda Mojiri-Forushani , Asma Mohammadi

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引用次数: 0

Abstract

The presence of pharmaceuticals in aquatic ecosystems and their impact on humans and the environment are growing concerns in environmental health. This study aimed to evaluate the potential reproductive effects of diclofenac, ibuprofen, and aspirin on dissociated ovarian and testicular cells from Arabian Sea bream, Acanthopagrus arabicus. The cells were exposed to varying concentrations of the pharmaceuticals for 48 h. Steroid (17-β-estradiol (E2) and 11-ketotestosterone (11-KT)) production by the cells was assessed at 0, 12, 24, and 48 h of the experiment. The findings showed that diclofenac did not impact the production of E2 and 11-KT by ovarian cells, but it did significantly decrease the secretion of 11-KT from testicular cells. Ibuprofen and aspirin, on the other hand, both increased the production of the studied steroid hormones by ovarian cells and reduced the secretion of 11-KT by testicular cells in a dose- and time-dependent manner. The pharmaceuticals studied were potent inhibitors of 11-KT secretion, particularly at higher concentrations in the cultured testicular cells. However, they were also found to stimulate steroid production from fish ovarian cells. In conclusion, the results suggest that analgesics (diclofenac, ibuprofen, and aspirin) have the potential to disrupt estrogen biosynthesis and impact reproduction in fish.

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水生生态系统中存在的药物及其对人类和环境的影响是环境健康领域日益关注的问题。本研究旨在评估双氯芬酸、布洛芬和阿司匹林对阿拉伯鲷离体卵巢和睾丸细胞的潜在生殖影响。将细胞暴露在不同浓度的药物中 48 小时。在实验的 0、12、24 和 48 小时评估细胞产生的类固醇（17-β-雌二醇（E2）和 11-酮睾酮（11-KT））。结果表明，双氯芬酸不会影响卵巢细胞产生 E2 和 11-KT，但会显著减少睾丸细胞分泌 11-KT。另一方面，布洛芬和阿司匹林都能增加卵巢细胞产生所研究的类固醇激素，并以剂量和时间依赖的方式减少睾丸细胞分泌 11-KT。所研究的药物是 11-KT 分泌的强效抑制剂，尤其是在培养的睾丸细胞中浓度较高时。不过，研究还发现，这些药物还能刺激鱼卵巢细胞分泌类固醇。总之，研究结果表明，镇痛药（双氯芬酸、布洛芬和阿司匹林）有可能破坏雌激素的生物合成，影响鱼类的繁殖。

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来源期刊

Toxicology in Vitro 医学-毒理学

CiteScore

6.50

自引率

3.10%

发文量

181

审稿时长

65 days

期刊介绍： Toxicology in Vitro publishes original research papers and reviews on the application and use of in vitro systems for assessing or predicting the toxic effects of chemicals and elucidating their mechanisms of action. These in vitro techniques include utilizing cell or tissue cultures, isolated cells, tissue slices, subcellular fractions, transgenic cell cultures, and cells from transgenic organisms, as well as in silico modelling. The Journal will focus on investigations that involve the development and validation of new in vitro methods, e.g. for prediction of toxic effects based on traditional and in silico modelling; on the use of methods in high-throughput toxicology and pharmacology; elucidation of mechanisms of toxic action; the application of genomics, transcriptomics and proteomics in toxicology, as well as on comparative studies that characterise the relationship between in vitro and in vivo findings. The Journal strongly encourages the submission of manuscripts that focus on the development of in vitro methods, their practical applications and regulatory use (e.g. in the areas of food components cosmetics, pharmaceuticals, pesticides, and industrial chemicals). Toxicology in Vitro discourages papers that record reporting on toxicological effects from materials, such as plant extracts or herbal medicines, that have not been chemically characterized.