The potential of alphapinene as a therapeutic agent for maternal hypoxia-induced cognitive impairments: a study on HO-1 and Nrf2 gene expression in rats.

IF 3.5 3区医学 Q2 ENDOCRINOLOGY & METABOLISM

Metabolic brain disease Pub Date : 2025-01-27 DOI:10.1007/s11011-024-01519-0

Mahsa Mahmoodi Atabaki, Zohreh Ghotbeddin, Kaveh Rahimi, Mohammad Reza Tabandeh

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Abstract

This research seeks to address the gap in past studies by examining the role of the Nrf2 (nuclear factor erythroid 2-related factor 2) and HO-1 (heme oxygenase-1) signaling pathways in hypoxia and the potential effects of alpha-pinene on these factors. Wistar rats were divided into 7 experimental groups (n = 7): 1) control, 2 and 3) groups receiving alpha-pinene 5 and 10 mg/kg (i.p., for 21 days), 4) hypoxia group (7% O2 and 93 N2, 3 h, GD14 to GD18), 5 and 6) groups receiving alpha-pinene 5 and 10 after hypoxia. Memory and cognition were evaluated using the Morris water maze and novel object recognition tests. Inflammation was assessed by ELISA method and Nrf2 and HO-1 Nrf2 gene expression was evaluated using real-time PCR in the hippocampus. Recognition index, spatial memory, and Nrf2-H0-1 gene expression significantly reduced in the hypoxia group compared to the control group, and alpha-pinene injection in the offspring improved cognition, memory, and Nrf2- HO-1 gene expression in the groups were affected by hypoxia. Inflammation factors in the hypoxia group were higher than the control, but alpha-pinene significantly decreased inflammation ( all cases p < 0.05). Based on the results, it seems that alpha-pinene prevents cognitive and memory loss by increasing Nrf2 and H0-1 gene expression and reducing inflammation.

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α蒎烯作为一种治疗母体缺氧所致认知障碍的药物的潜力：对大鼠HO-1和Nrf2基因表达的研究

本研究旨在通过研究Nrf2（核因子-红细胞2相关因子2）和HO-1（血红素加氧酶-1）信号通路在缺氧中的作用以及α -蒎烯对这些因子的潜在影响来弥补以往研究的空白。Wistar大鼠分为7个实验组（n = 7）： 1)对照组，2、3)α -蒎烯5、10 mg/kg（每日1次，持续21 d）组，4)缺氧组（7% O2和93 N2, 3 h, GD14 ~ GD18）， 5、6)缺氧后α -蒎烯5、10组。采用Morris水迷宫和新颖的物体识别测试评估记忆和认知能力。采用ELISA法检测大鼠海马组织炎症反应，real-time PCR检测海马组织Nrf2和HO-1 Nrf2基因表达。与对照组相比，缺氧组小鼠的识别指数、空间记忆和Nrf2- HO-1基因表达均显著降低，子代注射α -蒎烯可改善小鼠的认知、记忆，缺氧组小鼠Nrf2- HO-1基因表达均受到影响。缺氧组炎症因子高于对照组，但α -蒎烯显著降低炎症(所有病例p

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来源期刊

Metabolic brain disease 医学-内分泌学与代谢

CiteScore

5.90

自引率

5.60%

发文量

248

审稿时长

6-12 weeks

期刊介绍： Metabolic Brain Disease serves as a forum for the publication of outstanding basic and clinical papers on all metabolic brain disease, including both human and animal studies. The journal publishes papers on the fundamental pathogenesis of these disorders and on related experimental and clinical techniques and methodologies. Metabolic Brain Disease is directed to physicians, neuroscientists, internists, psychiatrists, neurologists, pathologists, and others involved in the research and treatment of a broad range of metabolic brain disorders.