Diffusion-weighted arterial spin labeling MRI to investigate mannitol-induced blood brain barrier disruption

IF 2.1 4区医学 Q2 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING

Magnetic resonance imaging Pub Date : 2025-01-24 DOI:10.1016/j.mri.2025.110335

Yash Vardhan Tiwari , Eric R. Muir , Zhao Jiang , Tim Q. Duong

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引用次数: 0

Abstract

Purpose

Diffusion-weighted arterial spin labeling (DW-ASL) MRI has been proposed to determine the rate of water exchange (K_w) across the blood brain barrier (BBB). This study aims to further evaluate K_w MRI by comparing it with standard dynamic contrast-enhanced (DCE) MRI and histology in association with mannitol-induced disruption of the BBB.

Methods

DW-ASL was measured using a multiple b-value MRI protocol in normal rats at three post-labeling delays (N = 19), before and after intra-carotid injection of mannitol to disrupt BBB in one hemisphere (N = 13). An approach using only two b-values to detect mannitol-induced changes was also tested. DCE MRI and Evans blue histology were performed on the same animals. Quantitative analysis and pixel-by-pixel correlation were performed amongst K_w, DCE MRI and Evans blue histology.

Results

K_w in the grey matter in the normal rat brain was 252 ± 38 min⁻¹ (±standard error of the mean). The two b-value approach provided reasonable approximation of multiple-b DW-ASL parameters, reducing acquisition time. K_w is sensitive to mannitol-induced changes in BBB permeability and was reduced to 89 ± 17 min⁻¹ in the affected hemisphere compared to 191 ± 22 min⁻¹ in the unaffected hemisphere (P < 0.05). Regions with abnormality in K_w maps were in general agreement with DCE and Evans blue maps, although there are some distinct differences in location and the change in values.

Conclusion

K_w is sensitive to mannitol-induced changes in the BBB, with BBB disruption confirmed by DCE MRI and Evans blue histology.

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弥散加权动脉自旋标记MRI研究甘露醇引起的血脑屏障破坏。

目的：提出了弥散加权动脉自旋标记（DW-ASL） MRI来确定血脑屏障（BBB）的水交换速率（Kw）。本研究旨在通过将Kw MRI与标准动态对比增强（DCE） MRI和与甘露醇引起的血脑屏障破坏相关的组织学进行比较，进一步评估Kw MRI。方法：正常大鼠在标记后延迟（N = 19）、颈动脉内注射甘露醇破坏一个半球血脑屏障（N = 13）之前和之后的三个时间（N = 19），采用多b值MRI方案测量DW-ASL。还测试了仅使用两个b值来检测甘露醇引起的变化的方法。对同一动物进行DCE MRI和Evans blue组织学检查。在Kw、DCE MRI和Evans蓝色组织学之间进行定量分析和逐像素相关性。结果：正常大鼠脑灰质Kw为252 ± 38 min-1（±标准误差平均值）。双b值法提供了对多个DW-ASL参数的合理逼近，减少了采集时间。Kw对甘露醇诱导的血脑屏障通透性变化很敏感，受影响半球的Kw降至89 ± 17 min-1，而未受影响半球的Kw降至191 ± 22 min-1 (P w图与DCE和Evans蓝图基本一致，尽管在位置和值的变化上存在一些明显的差异。结论：Kw对甘露醇诱导的血脑屏障改变敏感，DCE MRI和Evans蓝组织学证实血脑屏障破坏。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Magnetic resonance imaging 医学-核医学

CiteScore

4.70

自引率

4.00%

发文量

194

审稿时长

83 days

期刊介绍： Magnetic Resonance Imaging (MRI) is the first international multidisciplinary journal encompassing physical, life, and clinical science investigations as they relate to the development and use of magnetic resonance imaging. MRI is dedicated to both basic research, technological innovation and applications, providing a single forum for communication among radiologists, physicists, chemists, biochemists, biologists, engineers, internists, pathologists, physiologists, computer scientists, and mathematicians.