Transposition of the heat-activated retrotransposon ONSEN results in enhanced hypocotyl elongation.

IF 1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Genes & genetic systems Pub Date : 2025-03-28 Epub Date: 2025-01-25 DOI:10.1266/ggs.24-00110
Ryu Hasegawa, Hidetaka Ito
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引用次数: 0

Abstract

We aimed to identify new mutants resulting from ONSEN transposition in Arabidopsis thaliana by subjecting nrpd1 mutant seedlings to heat stress. We isolated a mutant with a significantly elongated hypocotyl, named Long hypocotyl in ONSEN-inserted line 1 (hyo1). This phenotype was heritable, with progeny consistently displaying longer hypocotyls than the wild type. Genetic analysis revealed that this trait was due to a single recessive mutation. Further mapping and sequencing identified the insertion of ONSEN into the HY2 gene, a crucial regulator of hypocotyl elongation. The insertion disrupted HY2 transcription, as confirmed by quantitative PCR, leading to the observed phenotype. To assess any influence of the nrpd1 background, we generated lines backcrossed twice to wild-type Col-0, and the results were consistent with those observed in the original mutant lines. Furthermore, we examined the effect of HY2 and HYO1 mutations on flowering time by analyzing the expression levels of FT. The hyo1 mutant exhibited earlier flowering compared to both wild type and the nrpd1 mutant, with increased FT expression levels. This research highlights the impact of ONSEN transposition on gene function and phenotypic variation in A. thaliana, providing new insights into the mutagenic potential of transposons and their role in shaping plant traits.

热激活的反转录转座子ONSEN的转位导致下胚轴伸长的变化。
在我们的研究中,我们旨在通过将nrpd1突变体置于热胁迫下,鉴定拟南芥中ONSEN转位产生的新突变体。我们分离到一个下胚轴明显延长的突变体,命名为“ONSEN插入系1中的长下胚轴”(HYO1)。这种表型是可遗传的,后代始终显示较长的下胚轴比野生型。遗传分析表明,该性状是由单隐性突变引起的。进一步的定位和测序确定了ONSEN在HY2基因中的插入,HY2基因是下胚轴伸长的关键调节因子。正如定量PCR证实的那样,插入破坏了HY2的转录,导致了观察到的表型。为了评估nrpd1背景的影响,我们制作了两次与野生型col0回交的系,结果与原始突变系的观察结果一致。此外,我们通过分析FT表达水平,研究了HY2和HYO1突变对开花时间的影响。与野生型和nrpd1突变体相比,HYO1突变体开花时间提前,FT表达水平增加。本研究强调了ONSEN转位对拟南芥基因功能和表型变异的重要影响,为转座子的诱变潜力及其在植物性状塑造中的作用提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Genes & genetic systems
Genes & genetic systems 生物-生化与分子生物学
CiteScore
1.50
自引率
0.00%
发文量
22
审稿时长
>12 weeks
期刊介绍: Genes & Genetic Systems , formerly the Japanese Journal of Genetics , is published bimonthly by the Genetics Society of Japan.
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