Evaluating the antioxidant, anti-inflammatory, and neuroprotective potential of fruiting body and mycelium extracts from edible yellow morel (Morchella esculenta L. Pers.).

IF 3.2 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Food Science Pub Date : 2025-01-01 DOI:10.1111/1750-3841.17619

Rida Haider, Luisa Agnello, Shahid Masood Shah, Muhammad Sufyan, Nimra Khan, Abdul Nazir, Marcello Ciaccio, Sidra Rehman

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Abstract

Scope: This study aimed to assess the antioxidant, anti-inflammatory, and acetylcholinesterase activities of fruiting bodies (FB) and mycelium (M) extracts of Morchella esculenta L. collected from various regions of Pakistan. The samples included Skardu fruiting body (SKFB) and mycelia Skardu (SKM), Malam Jaba fruiting body (MJFB) and Malam Jaba mycelia (MJM), Krair Mansehra fruiting body (KMFB) and Krair Mansehra mycelia (KMM), and Thandiani fruiting body (TFB) and Thandiani mycelia (TM).

Methods and results: The IC₅₀ values for free radical scavenging activity of all samples revealed that fruiting body SKFB and MJFB of M. esculenta are significantly involved in relieving oxidative stress. Bovine serum albumin conformation destruction assay showed a significantly increased anti-inflammatory activity of SKFB with an IC₅₀ value of 10.94 ± 0.098 µg/mL. The human red blood cell protection assay showed that TFB has a lower EC₅₀ value as compared to other samples. KMFB and KMM extracts of M. esculenta showed significantly higher anti-acetylcholinesterase activity compared to the standard drug, donepezil. The lower IC₅₀ value of M. esculenta extracts suggested higher efficacy for acetylcholinesterase (AChE) inhibition. Enzyme kinetics results showed that KMFB of M. esculenta is a competitive inhibitor, while KMM and Donepezil are noncompetitive AChE inhibitors. Further, molecular docking, physicochemical properties and ADMET analysis of M. esculenta constituents showed smooth drug diffusion and protection against neurodegenerative disorders.

Conclusion: This study indicates that M. esculenta extracts may hold significant therapeutic potential for neurodegenerative diseases, opening a path for potential therapeutic strategies.

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研究范围本研究旨在评估从巴基斯坦不同地区采集的Morchella esculenta L.子实体（FB）和菌丝体（M）提取物的抗氧化、抗炎和乙酰胆碱酯酶活性。样品包括 Skardu 子实体（SKFB）和菌丝体 Skardu（SKM）、Malam Jaba 子实体（MJFB）和 Malam Jaba 菌丝体（MJM）、Krair Mansehra 子实体（KMFB）和 Krair Mansehra 菌丝体（KMM）以及 Thandiani 子实体（TFB）和 Thandiani 菌丝体（TM）：所有样品清除自由基活性的 IC50 值都表明，M. esculenta 的子实体 SKFB 和 MJFB 在缓解氧化应激方面有显著作用。牛血清白蛋白构象破坏试验表明，SKFB 的抗炎活性明显增强，IC50 值为 10.94 ± 0.098 µg/mL。人红细胞保护试验表明，与其他样品相比，TFB 的 EC50 值较低。与标准药物多奈哌齐相比，M. esculenta 的 KMFB 和 KMM 提取物显示出更高的抗乙酰胆碱酯酶活性。M.esculenta提取物的IC50值较低，这表明其抑制乙酰胆碱酯酶（AChE）的功效较高。酶动力学结果表明，M. esculenta 的 KMFB 是一种竞争性抑制剂，而 KMM 和多奈哌齐则是非竞争性 AChE 抑制剂。此外，M. esculenta 成分的分子对接、理化性质和 ADMET 分析表明，药物扩散顺畅，对神经退行性疾病有保护作用：本研究表明，M. esculenta 提取物可能对神经退行性疾病具有显著的治疗潜力，为潜在的治疗策略开辟了道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Food Science 工程技术-食品科技

CiteScore

7.10

自引率

2.60%

发文量

412

审稿时长

3.1 months

期刊介绍： The goal of the Journal of Food Science is to offer scientists, researchers, and other food professionals the opportunity to share knowledge of scientific advancements in the myriad disciplines affecting their work, through a respected peer-reviewed publication. The Journal of Food Science serves as an international forum for vital research and developments in food science. The range of topics covered in the journal include: -Concise Reviews and Hypotheses in Food Science -New Horizons in Food Research -Integrated Food Science -Food Chemistry -Food Engineering, Materials Science, and Nanotechnology -Food Microbiology and Safety -Sensory and Consumer Sciences -Health, Nutrition, and Food -Toxicology and Chemical Food Safety The Journal of Food Science publishes peer-reviewed articles that cover all aspects of food science, including safety and nutrition. Reviews should be 15 to 50 typewritten pages (including tables, figures, and references), should provide in-depth coverage of a narrowly defined topic, and should embody careful evaluation (weaknesses, strengths, explanation of discrepancies in results among similar studies) of all pertinent studies, so that insightful interpretations and conclusions can be presented. Hypothesis papers are especially appropriate in pioneering areas of research or important areas that are afflicted by scientific controversy.