Genome-Wide microRNA Expression Profiling in Human Spermatozoa and Its Relation to Sperm Quality.

IF 2.8 3区生物学 Q2 GENETICS & HEREDITY

Genes Pub Date : 2025-01-04 DOI:10.3390/genes16010053

Nino-Guy Cassuto, Florence Boitrelle, Hakima Mouik, Lionel Larue, Gwenola Keromnes, Nathalie Lédée, Laura Part-Ellenberg, Geraldine Dray, Léa Ruoso, Alexandre Rouen, John De Vos, Said Assou

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引用次数: 0

Abstract

Background: Sperm samples are separated into bad and good quality samples in function of their phenotype, but this does not indicate their genetic quality.

Methods: Here, we used GeneChip miRNA arrays to analyze microRNA expression in ten semen samples selected based on high-magnification morphology (score 6 vs. score 0) to identify miRNAs linked to sperm phenotype.

Results: We found 86 upregulated and 21 downregulated miRNAs in good-quality sperm (score 6) compared with bad-quality sperm samples (score 0) (fold change > 2 and p-value < 0.05). MiR-34 (FC × 30, p = 8.43 × 10^-8), miR-30 (FC × 12, p = 3.75 × 10^-6), miR-122 (FC × 8, p = 0.0031), miR-20 (FC × 5.6, p = 0.0223), miR-182 (FC × 4.83, p = 0.0008) and miR-191 (FC × 4, p = 1.61 × 10^-6) were among these upregulated miRNAs. In silico prediction algorithms predicted that miRNAs upregulated in good-quality sperm targeted 910 genes involved in key biological functions of spermatozoa, such as cell death and survival, cellular movement, molecular transport, response to stimuli, metabolism, and the regulation of oxidative stress. Genes deregulated in bad-quality sperm were involved in cell growth and proliferation.

Conclusions: This study reveals that miRNA profiling may provide potential biomarkers of sperm quality.

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来源期刊

Genes GENETICS & HEREDITY-

CiteScore

5.20

自引率

5.70%

发文量

1975

审稿时长

22.94 days

期刊介绍： Genes (ISSN 2073-4425) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to genes, genetics and genomics. It publishes reviews, research articles, communications and technical notes. There is no restriction on the length of the papers and we encourage scientists to publish their results in as much detail as possible.