Prenatal lipopolysaccharide stimulation modulates gastrointestinal immunity and oxidative status in weaned pigs.

IF 3.9 3区 医学 Q1 GASTROENTEROLOGY & HEPATOLOGY
Ty M Mitchell, Nicole C Burdick Sanchez, Jeff A Carroll, Paul R Broadway, Jerrad F Legako, Brooke M Bowen, Amy L Petry
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引用次数: 0

Abstract

Gastrointestinal immunity and antioxidant defenses may be bolstered in young animals through prenatal immune stimulation (PIS), but this is largely uninvestigated in swine. This study tested the hypothesis that PIS could regulate offspring's gastrointestinal immune response and oxidative stress profile. To this end, a PIS model was utilized in sows, delivering low-dose lipopolysaccharide (LPS) during the final third of gestation to target the developing immune system. On day 78 ± 1.8 of gestation, 14 Camborough sows (parity = 2.6 ± 1.4) received either saline (Control, CON) or LPS from Escherichia coli O111:B4 (2.5 µg/kg of body wt). A subset of 34 weaned barrows (n = 17 CON, PIS), weaned at 21 ± 1.3 days, were anesthetized for subcutaneous temperature loggers and jugular catheter placement. Following recovery, all pigs received an intravenous injection of LPS (10 µg/kg·body wt) from E. coli O111:B4. Our findings demonstrate that PIS enhances the gut immune response by upregulating key inflammatory cytokines, indicative of a proinflammatory profile. Consistently across the jejunum and ileum, stem cell factor was modulated with heightened expression in PIS than CON (P ≤ 0.05). In the ileum alone, PIS exhibited heightened expression of proinflammatory cytokines and chemokines, including TNFα, IL-6, IL-1β, and CCL3L1, compared with CON (P ≤ 0.05). Exposure to PIS resulted in reduced systemic total antioxidant capacity at hours 2 and 4 postchallenge (P = 0.004). Piglets exposed to PIS had decreased jejunal tissue malondialdehyde concentrations (P = 0.049). Together, these data indicate that exposure to PIS alters the inflammatory profile of the gastrointestinal immune response and oxidative status in weaned pigs.NEW & NOTEWORTHY These studies represent novel investigations into the influence of prenatal immune stimulation (PIS) in swine on the gastrointestinal immune response and oxidative status of offspring following subsequent immune challenge. Notable alterations were observed in gut protein biomarkers, particularly the upregulation of proinflammatory cytokines TNFα, IL-6, and IL-1β in PIS-exposed pigs, but has variable effects on oxidative status. Altered intestinal immune development may contribute to an increased risk for inflammatory disease associated with prenatal immune stimulation.

产前脂多糖刺激调节断奶仔猪胃肠道免疫和氧化状态。
胃肠免疫和抗氧化防御可能通过产前免疫系统刺激(PIS)在幼龄动物中得到加强,但这在猪中基本上没有研究。本研究验证了PIS可以调节后代胃肠道免疫反应和氧化应激谱的假设。为此,在母猪中使用了PIS模型,在妊娠的最后三分之一提供低剂量的LPS以靶向发育中的免疫系统。在妊娠78±1.8天,14头坎伯勒母猪(胎次= 2.6±1.4)分别接受生理盐水(CON)和大肠杆菌O111:B4 LPS (2.5 μg/kg体重)。34只断奶仔猪(n=17 CON, PIS)在21±1.3天断奶,麻醉用于皮下温度记录仪和颈静脉导管放置。恢复后,所有猪静脉注射大肠杆菌O111:B4 LPS (10 μg/kg体重)。我们的研究结果表明,PIS通过上调关键的炎症细胞因子来增强肠道免疫反应,表明其具有促炎作用。在整个空肠和回肠中,SCF在PIS中的表达与CON一致上调(P≤0.05)。仅在回肠中,与CON相比,PIS的促炎细胞因子和趋化因子,包括TNFα、IL-6、IL-1β和CCL3L1的表达升高(P≤0.05)。暴露于PIS后2-4小时,全身总抗氧化能力(TAC)降低(P= 0.004)。仔猪暴露于PIS后,空肠组织丙二醛(MDA)浓度降低(P= 0.049)。综上所述,这些数据表明,暴露于PIS会改变断奶仔猪胃肠道免疫反应的炎症谱和氧化状态。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
9.40
自引率
2.20%
发文量
104
审稿时长
1 months
期刊介绍: The American Journal of Physiology-Gastrointestinal and Liver Physiology publishes original articles pertaining to all aspects of research involving normal or abnormal function of the gastrointestinal tract, hepatobiliary system, and pancreas. Authors are encouraged to submit manuscripts dealing with growth and development, digestion, secretion, absorption, metabolism, and motility relative to these organs, as well as research reports dealing with immune and inflammatory processes and with neural, endocrine, and circulatory control mechanisms that affect these organs.
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