Methylation of SOX1 and PAX1 Are Risk Factors and Potential Biomarkers for Cervical Lesions.

IF 2.1 Q3 ONCOLOGY

World Journal of Oncology Pub Date : 2025-02-01 Epub Date: 2024-12-31 DOI:10.14740/wjon1985

Yan Die Lin, Xiao Yue Li, Li Wei Shao, Ai Jun Liu

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引用次数: 0

Abstract

Background: The correlation between methylation of paired box gene 1 (PAX1) and sex determining region Y-box 1 (SOX1) with human papillomavirus (HPV) infection and the progression of cervical lesions is not well understood. This study aims to explore the potential value of PAX1 and SOX1 as diagnostic biomarkers for cervical diseases.

Methods: A total of 139 cervical biopsy tissue samples were obtained from the Department of Pathology, the Seventh Medical Center, Chinese PLA General Hospital from 2021 to 2023. The samples include 32 cases of chronic cervicitis (inflammation group), 30 cases of low-grade squamous intraepithelial lesions (LSIL group), 50 cases of high-grade squamous intraepithelial lesions (HSIL group), and 27 cases of cervical squamous cell carcinoma (CSCC group). DNA was extracted from paraffin-embedded tissues, and the levels of HPV infection and methylation of PAX1 and SOX1 were detected.

Results: The methylation index (M-index) of PAX1 and SOX1 in the HSIL and CSCC groups is significantly higher than in the inflammation group (both P < 0.0001), with no significant difference between the LSIL and inflammation groups. There is no significant difference in the positive PAX1 and SOX1 methylation rate with HPV infection and age. The positive rates of PAX1 methylation in the inflammation, LSIL, HSIL, and CSCC groups were 3.13%, 10.00%, 44.00%, and 88.89%, respectively. The positive rates of SOX1 methylation were 3.13%, 10.00%, 40.00%, and 77.78%, respectively, and increasing with the progression of cervical lesions (R² = 0.9189/R² = 0.9279, P < 0.0001/P < 0.0001). Comparing LSIL, HSIL, and CSCC with the inflammation group and using cervical biopsy pathology diagnosis as the gold standard, methylation of PAX1 and SOX1 is a risk factor for HSIL and CSCC, with odds ratio (OR) values significantly increasing as lesions progress. The sensitivity of PAX1 and SOX1 methylation to cervical lesions increases with the progression of the lesions.

Conclusions: Methylation of SOX1 and PAX1 is not associated with HPV infection. The positive rate of methylation for SOX1 and PAX1 is positively correlated with cervical lesions, which can serve as potential biomarkers for HSIL and CSCC. They are risk factors and potential screening indicators for HSIL and above cervical lesions.

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SOX1和PAX1甲基化是宫颈病变的危险因素和潜在的生物标志物。

背景：配对盒基因1 （PAX1）和性别决定区y -盒1 （SOX1）甲基化与人乳头瘤病毒（HPV）感染和宫颈病变进展的关系尚不清楚。本研究旨在探讨PAX1和SOX1作为宫颈疾病诊断生物标志物的潜在价值。方法：于2021 - 2023年在解放军总医院第七医学中心病病科采集宫颈活检组织标本139例。样本包括慢性宫颈炎32例（炎症组），低级别鳞状上皮内病变30例（LSIL组），高级别鳞状上皮内病变50例（HSIL组），宫颈鳞状细胞癌27例（CSCC组）。从石蜡包埋组织中提取DNA，检测HPV感染水平和PAX1、SOX1甲基化水平。结果：PAX1和SOX1的甲基化指数（M-index）在HSIL组和CSCC组均显著高于炎症组（P均< 0.0001），而LSIL组与炎症组间差异无统计学意义。PAX1和SOX1阳性甲基化率与HPV感染和年龄无显著差异。炎症组、LSIL组、HSIL组和CSCC组PAX1甲基化阳性率分别为3.13%、10.00%、44.00%和88.89%。SOX1甲基化阳性率分别为3.13%、10.00%、40.00%、77.78%，且随着宫颈病变的进展呈上升趋势（R2 = 0.9189/R2 = 0.9279, P < 0.0001/P < 0.0001）。将LSIL、HSIL和CSCC与炎症组进行比较，并以宫颈活检病理诊断为金标准，PAX1和SOX1甲基化是HSIL和CSCC的危险因素，随着病变进展，优势比（OR）值显著增加。PAX1和SOX1甲基化对宫颈病变的敏感性随着病变的进展而增加。结论：SOX1和PAX1的甲基化与HPV感染无关。SOX1和PAX1的甲基化阳性率与宫颈病变呈正相关，可作为HSIL和CSCC的潜在生物标志物。它们是HSIL及以上宫颈病变的危险因素和潜在筛查指标。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

World Journal of Oncology ONCOLOGY-

CiteScore

6.10

自引率

15.40%

发文量

期刊介绍： World Journal of Oncology, bimonthly, publishes original contributions describing basic research and clinical investigation of cancer, on the cellular, molecular, prevention, diagnosis, therapy and prognosis aspects. The submissions can be basic research or clinical investigation oriented. This journal welcomes those submissions focused on the clinical trials of new treatment modalities for cancer, and those submissions focused on molecular or cellular research of the oncology pathogenesis. Case reports submitted for consideration of publication should explore either a novel genomic event/description or a new safety signal from an oncolytic agent. The areas of interested manuscripts are these disciplines: tumor immunology and immunotherapy; cancer molecular pharmacology and chemotherapy; drug sensitivity and resistance; cancer epidemiology; clinical trials; cancer pathology; radiobiology and radiation oncology; solid tumor oncology; hematological malignancies; surgical oncology; pediatric oncology; molecular oncology and cancer genes; gene therapy; cancer endocrinology; cancer metastasis; prevention and diagnosis of cancer; other cancer related subjects. The types of manuscripts accepted are original article, review, editorial, short communication, case report, letter to the editor, book review.