In vitro culture of the parasitic stage larvae of hematophagous parasitic nematode Haemonchus contortus

IF 3.7 2区医学 Q1 PARASITOLOGY

International journal for parasitology Pub Date : 2025-01-21 DOI:10.1016/j.ijpara.2025.01.007

Lu Liu , Zongshan Zhang , Fuqiang Liu , Hui Liu , Lisha Ye , Feng Liu , Nishith Gupta , Chunqun Wang , Min Hu

{"title":"In vitro culture of the parasitic stage larvae of hematophagous parasitic nematode Haemonchus contortus","authors":"Lu Liu , Zongshan Zhang , Fuqiang Liu , Hui Liu , Lisha Ye , Feng Liu , Nishith Gupta , Chunqun Wang , Min Hu","doi":"10.1016/j.ijpara.2025.01.007","DOIUrl":null,"url":null,"abstract":"<div><div>Current research on common parasitic nematodes is limited because their infective stages cannot be propagated in vitro. Here, we report a culture system for developing L4s of <em>Haemonchus contortus</em>, a blood-feeding nematode of ruminants. Our results demonstrated that a proportionate mixture of NCTC-109 to Luria-Bertini (1:2) media promoted the formation of early L4s and then into late L4s upon inclusion of 12.5% (v/v) defibrinated blood, albeit with a decline in survival. Adding antioxidants (0.3 mg/mL of L-glutathione or 200 nmol of vitamin C) improved survival of L4s, with approximately 90% developing to late L4s by 22 days. These L4s showed parallel morphological features (such as digestive and reproduction systems) compared with in vivo L4s at day 7 (following challenge infection), although with delayed development. Our work optimized the in vitro culture system for L4s while providing an important platform for in-depth molecular research on <em>Haemonchus</em> and other related parasitic nematodes.</div></div>","PeriodicalId":13725,"journal":{"name":"International journal for parasitology","volume":"55 5","pages":"Pages 263-271"},"PeriodicalIF":3.7000,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7617482/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal for parasitology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0020751925000074","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PARASITOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Current research on common parasitic nematodes is limited because their infective stages cannot be propagated in vitro. Here, we report a culture system for developing L4s of Haemonchus contortus, a blood-feeding nematode of ruminants. Our results demonstrated that a proportionate mixture of NCTC-109 to Luria-Bertini (1:2) media promoted the formation of early L4s and then into late L4s upon inclusion of 12.5% (v/v) defibrinated blood, albeit with a decline in survival. Adding antioxidants (0.3 mg/mL of L-glutathione or 200 nmol of vitamin C) improved survival of L4s, with approximately 90% developing to late L4s by 22 days. These L4s showed parallel morphological features (such as digestive and reproduction systems) compared with in vivo L4s at day 7 (following challenge infection), although with delayed development. Our work optimized the in vitro culture system for L4s while providing an important platform for in-depth molecular research on Haemonchus and other related parasitic nematodes.

Abstract Image

查看原文本刊更多论文

噬血血线虫寄生期幼虫的体外培养。

目前对常见寄生线虫的研究是有限的，因为它们的感染阶段不能在体外繁殖。在这里，我们报告了一种培养系统，用于发展弯曲血线虫L4s，一种反刍动物的吸血线虫。我们的研究结果表明，NCTC-109与Luria-Bertini（1:2）培养基的比例混合物促进了早期L4s的形成，然后在12.5% （v/v）的去纤血中促进了晚期L4s的形成，尽管存活率下降。添加抗氧化剂（0.3 mg/mL的l -谷胱甘肽或200 nmol的维生素C）提高了L4s的存活率，大约90%的L4s在22天内发展到晚期。与体内L4s相比，这些L4s在第7天（感染后）表现出相似的形态特征（如消化和生殖系统），尽管发育迟缓。本研究优化了L4s的体外培养体系，为深入研究Haemonchus及其他相关寄生线虫的分子结构提供了重要平台。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

International journal for parasitology 医学-寄生虫学

CiteScore

8.40

自引率

2.50%

发文量

审稿时长

23 days

期刊介绍： International Journal for Parasitology offers authors the option to sponsor nonsubscriber access to their articles on Elsevier electronic publishing platforms. For more information please view our Sponsored Articles page. The International Journal for Parasitology publishes the results of original research in all aspects of basic and applied parasitology, including all the fields covered by its Specialist Editors, and ranging from parasites and host-parasite relationships of intrinsic biological interest to those of social and economic importance in human and veterinary medicine and agriculture.