The functional connotations of iron deficiency-effect on neutrophil oxidative burst activity in preschool children.

American journal of blood research Pub Date : 2024-12-25 eCollection Date: 2024-01-01 DOI:10.62347/TSPM9335
Shweta Dutt, Poonam Rani, Richa Gupta, Pooja Dewan, Mrinalini Kotru
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Abstract

Iron deficiency anaemia (IDA) makes an individual prone to bacterial infections. The antimicrobial defence mechanism of neutrophils is orchestrated by Nicotinamide Adenine Dinucleotide Phosphate Hydrogen (NADPH) oxidative burst which is iron-dependent. The few previous studies documenting a decrease in neutrophil oxidative burst in iron-deficient children have been based mainly on the Nitro blue tetrazolium test (NBT). Very few studies have been conducted using the more robust flow cytometry-based dihydro rhodamine (DHR) assay in this regard worldwide and none in India.

Aim: To estimate the effect of iron deficiency on neutrophil oxidative burst activity in children under 5 years of age by flow cytometry-based dihydro rhodamine (DHR) assay and compare it with the control group.

Methods: Thirty-six children between 6 months to 5 years of age diagnosed with moderate (Hb 7-10 gm/dl) to severe (Hb <7 gm/dl) iron deficiency anaemia were selected as cases with equal number of sex/age matched controls. The peripheral blood was analyzed for hematological and biochemical parameters such as complete iron profile, serum vitamin B12, and folate levels. The oxidative burst activity of neutrophils in peripheral blood was assessed using a flow-cytometry-based Dihydrorhodamine (DHR) assay.

Results: The percentage of neutrophils showing stimulation, Mean Fluorescence Index in stimulated neutrophils, and Neutrophil oxidative index (NOI) were significantly reduced in iron deficiency anaemia patients as compared to controls. In cases, haemoglobin showed significant positive correlation with NOI and percentage of neutrophils showing stimulation.

Conclusion: To conclude, a significant decrease in neutrophil oxidative burst parameters depicts an insufficient innate immune response to pathogens and makes Iron deficiency anaemia patients more susceptible to infections, further aggravated by the severity of anaemia.

缺铁的功能内涵——对学龄前儿童中性粒细胞氧化爆发活性的影响。
缺铁性贫血(IDA)使个体容易发生细菌感染。嗜中性粒细胞的抗微生物防御机制是由烟酰胺腺嘌呤二核苷酸磷酸氢(NADPH)的氧化爆发调控的,这是铁依赖性的。先前记录缺铁儿童中性粒细胞氧化爆发减少的少数研究主要基于硝基蓝四氮唑试验(NBT)。在这方面,世界范围内使用更强大的基于流式细胞术的二氢罗丹明(DHR)测定法进行的研究很少,印度没有。目的:通过流式细胞术检测二氢罗丹明(DHR),探讨缺铁对5岁以下儿童中性粒细胞氧化爆发活性的影响,并与对照组进行比较。方法:36名6个月至5岁之间诊断为中度(Hb 7-10 gm/dl)至重度(Hb)的儿童。结果:与对照组相比,缺铁性贫血患者显示刺激的中性粒细胞百分比、受刺激中性粒细胞的平均荧光指数和中性粒细胞氧化指数(NOI)显着降低。在某些情况下,血红蛋白与NOI和中性粒细胞百分比呈显著正相关。结论:综上所述,中性粒细胞氧化爆发参数的显著降低说明先天免疫对病原体的反应不足,使缺铁性贫血患者更容易感染,并因贫血的严重程度而进一步加重。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
American journal of blood research
American journal of blood research MEDICINE, RESEARCH & EXPERIMENTAL-
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