The Dynamic Changes of COL11A1 Expression During the Carcinogenesis and Development of Breast Cancer and as a Candidate Diagnostic and Prognostic Marker

IF 1.9 4区 医学 Q3 OBSTETRICS & GYNECOLOGY
Breast Journal Pub Date : 2025-01-09 DOI:10.1155/tbj/7861864
Yuli Wang, Jing Wang
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引用次数: 0

Abstract

Purpose: Collagen type XI alpha 1 (COL11A1), a critical member of the collagen superfamily, is essential for tissue structure and integrity. This study aimed to validate previously identified variations in COL11A1 expression during breast cancer carcinogenesis and progression, as well as elucidate their clinical implications.

Methods: COL11A1 mRNA expression levels were assessed using real-time reverse transcription-PCR (RT-PCR) in 30 pairs of normal breast tissue and primary breast cancer, 30 pairs of primary breast cancer and lymph node metastases, 30 benign tumors, and 107 primary breast cancers. COL11A1 protein expression was evaluated by Western blot in six matched trios of normal tissue, primary cancer, and lymph node metastasis.

Results: COL11A1 mRNA levels were significantly higher in primary breast cancer tissues (n = 30) than in adjacent normal breast tissues (p < 0.001). Conversely, lymph node metastases (n = 30) showed significantly lower COL11A1 mRNA levels compared to their primary breast cancer counterparts (p = 0.005). In a larger cohort, primary breast cancers (n = 107) had significantly elevated COL11A1 mRNA levels relative to adjacent normal tissues (n = 30) and benign tumors (n = 30) (p < 0.001). Benign tumors also demonstrated higher levels compared to normal tissues (p = 0.012). The protein expression patterns were consistent with the mRNA findings. Receiver operating characteristic (ROC) curve analysis confirmed the diagnostic relevance of COL11A1 expression levels. Significant associations were found between COL11A1 mRNA levels and clinical parameters including lymph node involvement (p = 0.046), clinical stage (p = 0.004), and progesterone receptor status (p = 0.048). Overexpression of COL11A1 was correlated with poor prognosis.

Conclusions: COL11A1 expression varies during breast tumor initiation and progression, with elevated levels linked to worse prognoses. These findings underscore COL11A1’s potential as a biomarker in breast cancer, suggesting its assessment could enhance diagnostic and prognostic strategies for more personalized patient management.

Abstract Image

乳腺癌发生发展过程中COL11A1表达的动态变化及其作为候选诊断和预后指标的研究
目的:胶原型XI α 1 (COL11A1)是胶原超家族的重要成员,对组织结构和完整性至关重要。本研究旨在验证先前发现的COL11A1在乳腺癌发生和发展过程中的表达变化,并阐明其临床意义。方法:采用实时逆转录- pcr (RT-PCR)技术,对30对正常乳腺组织和原发性乳腺癌、30对原发性乳腺癌和淋巴结转移、30例良性肿瘤和107例原发性乳腺癌患者中COL11A1 mRNA的表达水平进行检测。Western blot检测了COL11A1蛋白在正常组织、原发癌和淋巴结转移的6个配对组中的表达。结果:COL11A1 mRNA水平在原发性乳腺癌组织(n = 30)中显著高于邻近正常乳腺组织(p < 0.001)。相反,淋巴结转移(n = 30)与原发性乳腺癌相比,COL11A1 mRNA水平显著降低(p=0.005)。在一个更大的队列中,原发性乳腺癌(n = 107)与邻近正常组织(n = 30)和良性肿瘤(n = 30)相比COL11A1 mRNA水平显著升高(p < 0.001)。与正常组织相比,良性肿瘤也显示出更高的水平(p=0.012)。蛋白质表达模式与mRNA的发现一致。受试者工作特征(ROC)曲线分析证实COL11A1表达水平与诊断相关。COL11A1 mRNA水平与临床参数,包括淋巴结累及(p=0.046)、临床分期(p=0.004)和孕激素受体状态(p=0.048)之间存在显著相关性。COL11A1过表达与预后不良相关。结论:COL11A1的表达在乳腺肿瘤的发生和发展过程中发生变化,其表达水平升高与预后不良有关。这些发现强调了COL11A1作为乳腺癌生物标志物的潜力,表明其评估可以增强诊断和预后策略,以实现更个性化的患者管理。
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来源期刊
Breast Journal
Breast Journal 医学-妇产科学
CiteScore
4.00
自引率
0.00%
发文量
47
审稿时长
4-8 weeks
期刊介绍: The Breast Journal is the first comprehensive, multidisciplinary source devoted exclusively to all facets of research, diagnosis, and treatment of breast disease. The Breast Journal encompasses the latest news and technologies from the many medical specialties concerned with breast disease care in order to address the disease within the context of an integrated breast health care. This editorial philosophy recognizes the special social, sexual, and psychological considerations that distinguish cancer, and breast cancer in particular, from other serious diseases. Topics specifically within the scope of The Breast Journal include: Risk Factors Prevention Early Detection Diagnosis and Therapy Psychological Issues Quality of Life Biology of Breast Cancer.
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