Cycloastragenol promotes dorsal column axon regeneration in mice.

IF 4.2 3区医学 Q2 NEUROSCIENCES

Frontiers in Cellular Neuroscience Pub Date : 2025-01-03 eCollection Date: 2024-01-01 DOI:10.3389/fncel.2024.1424137

Tao Zihan, Teng Wenwen, Ma Yanxia, Saijilafu

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引用次数: 0

Abstract

Introduction: Cycloastragenol (CAG) has a wide range of pharmacological effects, including anti-inflammatory, antiaging, antioxidative, and antitumorigenic properties. In addition, our previous study showed that CAG administration can promote axonal regeneration in peripheral neurons. However, whether CAG can activate axon regeneration central nervous system (CNS) remains unknown.

Methods: Here, we established a novel mouse model for visualizing spinal cord dorsal column axon regeneration involving the injection of AAV2/9-Cre into the lumbar 4/5 dorsal root ganglion (DRG) of Rosa-tdTomato reporter mice. We then treated mice by intraperitoneal administration of CAG.

Results: Our results showed that intraperitoneal CAG injections significantly promoted the growth of vitro-cultured DRG axons as well as the growth of dorsal column axons over the injury site in spinal cord injury (SCI) mice. Our results further indicate that CAG administration can promote the recovery of sensory and urinary function in SCI mice.

Conclusion: Together, our findings highlight the therapeutic potential of CAG in spinal cord injury repair.

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环黄芪醇促进小鼠背柱轴突再生。

环黄芪醇（CAG）具有广泛的药理作用，包括抗炎、抗衰老、抗氧化和抗肿瘤特性。此外，我们之前的研究表明，CAG可以促进周围神经元轴突再生。然而，CAG是否能激活中枢神经系统的轴突再生尚不清楚。方法：通过向Rosa-tdTomato报告小鼠腰椎4/5背根神经节（DRG）注射AAV2/9-Cre，建立了一种新的小鼠脊髓背柱轴突再生模型。然后给小鼠腹腔注射CAG。结果：我们的研究结果显示，腹腔注射CAG可显著促进体外培养的脊髓损伤小鼠DRG轴突的生长以及脊髓损伤部位背柱轴突的生长。本研究结果进一步表明，CAG能促进脊髓损伤小鼠感觉功能和泌尿功能的恢复。结论：综上所述，我们的研究结果突出了CAG在脊髓损伤修复中的治疗潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Cellular Neuroscience NEUROSCIENCES-

CiteScore

7.90

自引率

3.80%

发文量

627

审稿时长

6-12 weeks

期刊介绍： Frontiers in Cellular Neuroscience is a leading journal in its field, publishing rigorously peer-reviewed research that advances our understanding of the cellular mechanisms underlying cell function in the nervous system across all species. Specialty Chief Editors Egidio D‘Angelo at the University of Pavia and Christian Hansel at the University of Chicago are supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.